ELK1 is a transcription factor and a member of the Ets family of transcription factors. Elk1 is a downstream transcription factor of the Ras-Raf-MEK-Erk (canonical MAPK) pathway.
Product Information
Format
Culture Supernatant
Control
A431 whole cell lysate.
Presentation
Unpurified rabbit monoclonal IgG in buffer contaiing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Anti-Elk1 Antibody, (C-term), clone E277, rabbit is an antibody against Elk1 for use in IC, IH, IH(P), IP & WB.
Key Applications
Western Blotting
Immunocytochemistry
Immunohistochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Immunohistochemistry: 1:50 dilution from a previous lot was used on immunohistochemistry.
Immunocytochemistry: 1:100 dilution from a previous lot was used on immunocytochemistry.
Immunoprecipitation: 1:80 dilution from a previous lot was used on immunoprecipitation.
Biological Information
Immunogen
KLH conjugated linear peptide of Elk-1.
Epitope
C-terminus
Clone
E277
Host
Rabbit
Specificity
This antibody recognizes Elk-1 at the C-Terminus. The antibody does not cross-react with other ETS family members.
Isotype
IgG
Species Reactivity
Human
Species Reactivity Note
Proven to react with human. Not expected to react with mouse and rat.
This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Function:Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element. Subunit structure:Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity. Ref.12 Subcellular location:Nucleus. Tissue specificity:Lung and testis. Post-translational modification:Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Ref.7 Sequence similarities:Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on A431 whole cell lysate.
Western Blot Analysis:
A 1:500 dilution of this lot was used to detect Elk-1 on A431 whole cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
ELK1 is a transcription factor and a member of the Ets family of transcription factors. Elk1 is a downstream transcription factor of the Ras-Raf-MEK-Erk (canonical MAPK) pathway.
Product Information
Format
Culture Supernatant
Control
A431 whole cell lysate.
Presentation
Unpurified rabbit monoclonal IgG in buffer contaiing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Anti-Elk1 Antibody, (C-term), clone E277, rabbit is an antibody against Elk1 for use in IC, IH, IH(P), IP & WB.
Key Applications
Western Blotting
Immunocytochemistry
Immunohistochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Immunohistochemistry: 1:50 dilution from a previous lot was used on immunohistochemistry.
Immunocytochemistry: 1:100 dilution from a previous lot was used on immunocytochemistry.
Immunoprecipitation: 1:80 dilution from a previous lot was used on immunoprecipitation.
Biological Information
Immunogen
KLH conjugated linear peptide of Elk-1.
Epitope
C-terminus
Clone
E277
Host
Rabbit
Specificity
This antibody recognizes Elk-1 at the C-Terminus. The antibody does not cross-react with other ETS family members.
Isotype
IgG
Species Reactivity
Human
Species Reactivity Note
Proven to react with human. Not expected to react with mouse and rat.
This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Function:Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element. Subunit structure:Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity. Ref.12 Subcellular location:Nucleus. Tissue specificity:Lung and testis. Post-translational modification:Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Ref.7 Sequence similarities:Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on A431 whole cell lysate.
Western Blot Analysis:
A 1:500 dilution of this lot was used to detect Elk-1 on A431 whole cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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