Myc is a member of the helix-loop-helix-leucine zipper (bHLH-LZ) family of transcription factors, which dimerizes with Max to activate transcription of some genes and repress others. As Max is essential for Myc activity, the availability of Max is a regulatory mechanism for Myc activity, and members of the Mad family of proteins compete with Myc for Max.
Product Information
Format
Culture Supernatant
Control
Calyculin/Okadaic acid-treted A431 lysate
Presentation
100 μL of rabbit monoclonal IgG in 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Applications
Application
Anti-phospho-c-Myc (Thr58/Ser62) Antibody is an antibody against phospho-c-Myc (Thr58/Ser62) for use in IF, IP & WB.
Key Applications
Western Blotting
Immunofluorescence
Immunoprecipitation
Application Notes
Suggested dilutions:
Western Blot: 1:10,000
Immunocytochemistry: 1:50-1:100
Immunoprecipitation: 1:100
Biological Information
Immunogen
Synthetic peptide corresponding to sequence surrounding and including phosphorylated Thr58 and Ser62 of human c-Myc.
Epitope
phosphorylated Thr58/Ser62
Host
Rabbit
Specificity
c-Myc when phosphorylated on residues Thr58/Ser62.
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
FUNCTION:SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. SIZE:439 amino acids; 48804 Da SUBUNIT:Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B. SUBCELLULAR LOCATION:Nucleus. PTM:Phosphorylated by PRKDC. DISEASE:SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1. SIMILARITY:SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Myc is a member of the helix-loop-helix-leucine zipper (bHLH-LZ) family of transcription factors, which dimerizes with Max to activate transcription of some genes and repress others. As Max is essential for Myc activity, the availability of Max is a regulatory mechanism for Myc activity, and members of the Mad family of proteins compete with Myc for Max.
Product Information
Format
Culture Supernatant
Control
Calyculin/Okadaic acid-treted A431 lysate
Presentation
100 μL of rabbit monoclonal IgG in 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Applications
Application
Anti-phospho-c-Myc (Thr58/Ser62) Antibody is an antibody against phospho-c-Myc (Thr58/Ser62) for use in IF, IP & WB.
Key Applications
Western Blotting
Immunofluorescence
Immunoprecipitation
Application Notes
Suggested dilutions:
Western Blot: 1:10,000
Immunocytochemistry: 1:50-1:100
Immunoprecipitation: 1:100
Biological Information
Immunogen
Synthetic peptide corresponding to sequence surrounding and including phosphorylated Thr58 and Ser62 of human c-Myc.
Epitope
phosphorylated Thr58/Ser62
Host
Rabbit
Specificity
c-Myc when phosphorylated on residues Thr58/Ser62.
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
FUNCTION:SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. SIZE:439 amino acids; 48804 Da SUBUNIT:Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B. SUBCELLULAR LOCATION:Nucleus. PTM:Phosphorylated by PRKDC. DISEASE:SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1. SIMILARITY:SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
