MDM4 (sometimes referred to as MDMX) is a member of the MDM family proteins. It is known to inhibit p53 and p73 mediated cell cycle arrest and apoptosis. MDM4 is amplified or overexpressed in 10-20% of tumors and 65% of retinoblastomas. The full-length protein has significant homology to MDM2 and can also bind to and inhibit p53. In both MDM2 and MDM4, this interaction with p53 occurs via a binding domain located in the N-terminal region of the protein. Other similar domains include the C-terminal RING finger motif. Although these proteins share similar domains, MDM2 and MDM4 are non-redundant p53 inhibitors, since normal levels of either protein does not compensate for the loss of the other. Recent evidence suggests complementary activities for these proteins with MDM4 regulating p53 activity, and MDM2 playing a key role in p53 stability.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Use Anti-MDM4 Antibody, clone 7A8 (mouse monoclonal antibody) validated in WB to detect MDM4 also known as Double minute 4 protein, MDM4-related protein 1, Mdm2-like p53-binding protein, Mdm4 p53 binding protein homolog (mouse).
Key Applications
Western Blotting
Biological Information
Immunogen
His-tagged recombinant protein corresponding to mouse MDM4.
Epitope
Unknown
Clone
7A8
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: Inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions. The short isoform is a more potent inhibitor of p53 activity than the long isoform.
SUBUNIT STRUCTURE: Binds to p53, p73 and MDM2.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: In all tissues tested. The short isoform is expressed in a variety of transformed cell lines.
DOMAIN: Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
SEQUENCE SIMILARITIES: Belongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected MDM4 in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
MDM4 (sometimes referred to as MDMX) is a member of the MDM family proteins. It is known to inhibit p53 and p73 mediated cell cycle arrest and apoptosis. MDM4 is amplified or overexpressed in 10-20% of tumors and 65% of retinoblastomas. The full-length protein has significant homology to MDM2 and can also bind to and inhibit p53. In both MDM2 and MDM4, this interaction with p53 occurs via a binding domain located in the N-terminal region of the protein. Other similar domains include the C-terminal RING finger motif. Although these proteins share similar domains, MDM2 and MDM4 are non-redundant p53 inhibitors, since normal levels of either protein does not compensate for the loss of the other. Recent evidence suggests complementary activities for these proteins with MDM4 regulating p53 activity, and MDM2 playing a key role in p53 stability.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Use Anti-MDM4 Antibody, clone 7A8 (mouse monoclonal antibody) validated in WB to detect MDM4 also known as Double minute 4 protein, MDM4-related protein 1, Mdm2-like p53-binding protein, Mdm4 p53 binding protein homolog (mouse).
Key Applications
Western Blotting
Biological Information
Immunogen
His-tagged recombinant protein corresponding to mouse MDM4.
Epitope
Unknown
Clone
7A8
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: Inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions. The short isoform is a more potent inhibitor of p53 activity than the long isoform.
SUBUNIT STRUCTURE: Binds to p53, p73 and MDM2.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: In all tissues tested. The short isoform is expressed in a variety of transformed cell lines.
DOMAIN: Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
SEQUENCE SIMILARITIES: Belongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected MDM4 in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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