ETS1 belongs to a family of transcription factors that regulates expression of several genes participating in extracellular matrix remodeling. Members of the ETS family regulate a variety of genes involved in stem cell development, cell senescence, and tumorigenesis. ETS1 is typically absent in healthy gastric epithelium, but expressed in many gastric carcinomas and oral squamus cell carcinomas.
This family of transcription factors contains 2 main domains; a transactivation domain (TA), and a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes. This DNA-Binding-Domain is highly conserved among ETS family members.
Product Information
Format
Ascites
Control
HL60 cell lysate
Presentation
Unpurified mouse monoclonal IgG1κ in preservative-free ascites.
Applications
Application
Use Anti-ETS1 DBD Antibody, clone 1ET 7F2 (Mouse Monoclonal Antibody) validated in WB to detect ETS1 DBD also known as Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1, ets protein.
Key Applications
Western Blotting
Biological Information
Immunogen
Linear peptide corresponding to human ETS1 DBD.
Epitope
Unknown
Clone
clone 1ET 7F2
Host
Mouse
Specificity
This antibody recognizes ETS1.
Isotype
IgG1κ
Species Reactivity
Rat Human Mouse
Species Reactivity Note
Demonstrated to react with human. Predicted to react with mouse and rat based on 100% sequence homology.
ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM].
PTM: Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity (By similarity). & Ubiquitinated; which induces proteasomal degradation (By similarity).
DISEASE: ETS is responsible for erythroblast and fibroblast transformation. The juxtaposition of the interferon and c-ETS-1 proto-oncogene may be involved in the pathogenesis of human monocytic leukemia.
SIMILARITY: Belongs to the ETS family. & Contains 1 ETS DNA-binding domain. & Contains 1 PNT (pointed) domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HL60 cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected ETS1 DBD on 10 µg of HL60 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
ETS1 belongs to a family of transcription factors that regulates expression of several genes participating in extracellular matrix remodeling. Members of the ETS family regulate a variety of genes involved in stem cell development, cell senescence, and tumorigenesis. ETS1 is typically absent in healthy gastric epithelium, but expressed in many gastric carcinomas and oral squamus cell carcinomas.
This family of transcription factors contains 2 main domains; a transactivation domain (TA), and a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes. This DNA-Binding-Domain is highly conserved among ETS family members.
Product Information
Format
Ascites
Control
HL60 cell lysate
Presentation
Unpurified mouse monoclonal IgG1κ in preservative-free ascites.
Applications
Application
Use Anti-ETS1 DBD Antibody, clone 1ET 7F2 (Mouse Monoclonal Antibody) validated in WB to detect ETS1 DBD also known as Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1, ets protein.
Key Applications
Western Blotting
Biological Information
Immunogen
Linear peptide corresponding to human ETS1 DBD.
Epitope
Unknown
Clone
clone 1ET 7F2
Host
Mouse
Specificity
This antibody recognizes ETS1.
Isotype
IgG1κ
Species Reactivity
Rat Human Mouse
Species Reactivity Note
Demonstrated to react with human. Predicted to react with mouse and rat based on 100% sequence homology.
ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM].
PTM: Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity (By similarity). & Ubiquitinated; which induces proteasomal degradation (By similarity).
DISEASE: ETS is responsible for erythroblast and fibroblast transformation. The juxtaposition of the interferon and c-ETS-1 proto-oncogene may be involved in the pathogenesis of human monocytic leukemia.
SIMILARITY: Belongs to the ETS family. & Contains 1 ETS DNA-binding domain. & Contains 1 PNT (pointed) domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HL60 cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected ETS1 DBD on 10 µg of HL60 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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