Vitamin D receptor (VDR) is a member of the nuclear receptor family of transcription factors. Upon activation by vitamin D, the VDR forms a heterodimer with the retinoid-X receptor and binds to hormone response elements on DNA resulting in expression or transrepression of specific gene products. In humans, the vitamin D receptor is encoded by the VDR gene. This receptor also functions as a receptor for the secondary bile acid, lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows similarity of sequence to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer.
Product Information
Format
Purified
Control
MCF7 cell lysate
Presentation
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl and 0.05% sodium azide.
Applications
Application
Detect Vitamin D receptor using this Anti-Vitamin D receptor Antibody, clone 9A7 validated for use in WB, IH(P) & IP.
FUNCTION:Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Plays a central role in calcium homeostasis.
SUBUNIT STRUCTURE:Homodimer in the absence of bound vitamin D3. Heterodimer with RXRA after vitamin D3 binding. Interacts with SMAD3. Interacts with MED1, NCOA1, NCOA2, NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes.
SUBCELLULAR LOCATION:Nucleus.
DOMAIN:Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
POLYMORPHISM:Genetic variations in VDR may determine Mycobacterium tuberculosis susceptibility [MIM:607948].
INVOLVEMENT IN DISEASE:Defects in VDR are the cause of type IIA rickets [MIM:277440]; also known as hypocalcemic vitamin D-resistant rickets (HVDRR). HVDRR is most frequently an autosomal recessive disorder characterized by severe rickets, hypocalcemia and secondary hyperparathyroidism.
SEQUENCE SIMILARITIES:Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
CAUTION:It is uncertain whether Met-1 or Met-4 is the initiator.
SEQUENCE CAUTION:The sequence AAP88938.1 differs from that shown. Reason: Erroneous gene model prediction.
Product Usage Statements
Quality Assurance
Evaluated by western blot on MCF7 cell lysate.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected vitamin D receptor in MCF7 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
Vrt, H
FUNC, WB, IH(P), IP
R
Purified
Monoclonal Antibody
Description
Catalogue Number
04-1526
Replaces
MAB1360
Description
Anti-Vitamin D receptor Antibody, clone 9A7
Alternate Names
vitamin D (1,25-dihydroxyvitamin D3) receptor
1,25-dihydroxyvitamin D3 receptor
Nuclear receptor subfamily 1 group I member 1
vitamin D (1,25- dihydroxyvitamin D3) receptor
Background Information
Vitamin D receptor (VDR) is a member of the nuclear receptor family of transcription factors. Upon activation by vitamin D, the VDR forms a heterodimer with the retinoid-X receptor and binds to hormone response elements on DNA resulting in expression or transrepression of specific gene products. In humans, the vitamin D receptor is encoded by the VDR gene. This receptor also functions as a receptor for the secondary bile acid, lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows similarity of sequence to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer.
Product Information
Format
Purified
Control
MCF7 cell lysate
Presentation
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl and 0.05% sodium azide.
Applications
Application
Detect Vitamin D receptor using this Anti-Vitamin D receptor Antibody, clone 9A7 validated for use in WB, IH(P) & IP.
FUNCTION:Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Plays a central role in calcium homeostasis.
SUBUNIT STRUCTURE:Homodimer in the absence of bound vitamin D3. Heterodimer with RXRA after vitamin D3 binding. Interacts with SMAD3. Interacts with MED1, NCOA1, NCOA2, NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes.
SUBCELLULAR LOCATION:Nucleus.
DOMAIN:Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
POLYMORPHISM:Genetic variations in VDR may determine Mycobacterium tuberculosis susceptibility [MIM:607948].
INVOLVEMENT IN DISEASE:Defects in VDR are the cause of type IIA rickets [MIM:277440]; also known as hypocalcemic vitamin D-resistant rickets (HVDRR). HVDRR is most frequently an autosomal recessive disorder characterized by severe rickets, hypocalcemia and secondary hyperparathyroidism.
SEQUENCE SIMILARITIES:Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
CAUTION:It is uncertain whether Met-1 or Met-4 is the initiator.
SEQUENCE CAUTION:The sequence AAP88938.1 differs from that shown. Reason: Erroneous gene model prediction.
Product Usage Statements
Quality Assurance
Evaluated by western blot on MCF7 cell lysate.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected vitamin D receptor in MCF7 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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