The Kinase Suppressor of Ras (KSR1) is a recently identified participant in the Ras signaling pathway. KSR is capable of uncoupling the MAP kinase activation from its target phosphorylation, and thus provide a novel mechanism for modulating the Ras-MAP kinase signaling pathway. The recently identified, B-KSR, is a related isoform. KSR is thought to phosphorylate and activate Raf-1, protecting against cytokine-mediated apoptosis. Recent studies suggest that KSR interacts with several components of the MAP kinase pathway, including Raf-1, MEK1/2 and ERK1/2. KSR seems to act as a scaffolding protein that coordinates the assembly of a membrane-localized, multiprotein MAP kinase complex, a vital step in Ras-mediated signal transduction.
Product Information
Format
Unpurified
Control
293 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-KSR1 Antibody, clone EPR2421Y, Rabbit is validated for use in WB, IC, IH(P) for the detection of KSR1.
Key Applications
Immunohistochemistry (Paraffin)
Western Blotting
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected was used in IHC.
Immunocytochemistry Analysis: A 1:50 dilution from a previous lot was used in IC.
Biological Information
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human KSR1.
This gene is a necessary element in receptor tyrosine kinase pathways, possibly as a tyrosine phosphorylation target. It is involved in regulation of RAF in the MAPK pathway and may also play a role in a MAPK-independent pathway.
FUNCTION:Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity. SUBUNIT STRUCTURE:Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner By similarity. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. SUBCELLULAR LOCATION:Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity. Note: In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery By similarity. PTM:Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated By similarity. SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on 293 cell lysates.
Western Blot Analysis: A 1:500-2,000 dilution of this antibody was used to detect KSR1 in 293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
The Kinase Suppressor of Ras (KSR1) is a recently identified participant in the Ras signaling pathway. KSR is capable of uncoupling the MAP kinase activation from its target phosphorylation, and thus provide a novel mechanism for modulating the Ras-MAP kinase signaling pathway. The recently identified, B-KSR, is a related isoform. KSR is thought to phosphorylate and activate Raf-1, protecting against cytokine-mediated apoptosis. Recent studies suggest that KSR interacts with several components of the MAP kinase pathway, including Raf-1, MEK1/2 and ERK1/2. KSR seems to act as a scaffolding protein that coordinates the assembly of a membrane-localized, multiprotein MAP kinase complex, a vital step in Ras-mediated signal transduction.
Product Information
Format
Unpurified
Control
293 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-KSR1 Antibody, clone EPR2421Y, Rabbit is validated for use in WB, IC, IH(P) for the detection of KSR1.
Key Applications
Immunohistochemistry (Paraffin)
Western Blotting
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected was used in IHC.
Immunocytochemistry Analysis: A 1:50 dilution from a previous lot was used in IC.
Biological Information
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human KSR1.
This gene is a necessary element in receptor tyrosine kinase pathways, possibly as a tyrosine phosphorylation target. It is involved in regulation of RAF in the MAPK pathway and may also play a role in a MAPK-independent pathway.
FUNCTION:Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity. SUBUNIT STRUCTURE:Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner By similarity. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. SUBCELLULAR LOCATION:Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity. Note: In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery By similarity. PTM:Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated By similarity. SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on 293 cell lysates.
Western Blot Analysis: A 1:500-2,000 dilution of this antibody was used to detect KSR1 in 293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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