ErbB2/Her2 is a member of the ErbB receptor family of tyrosine kinases. ErbB2 can be activated without a ligand if it is overexpressed and can be detected in approximately 40% of human breast cancers. ErbB2 is a major target that is being explored In the treatment of breast cancer and other carcinomas.
Product Information
Format
Unpurified
Control
SKBR-3 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-ErbB2/Her2 Antibody, clone EP1045Y, Rabbit is validated for use in WB, FC, IH(P), IP for the detection of ErbB2/Her2.
Key Applications
Immunoprecipitation
Western Blotting
Flow Cytometry
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected ErbB2/Her2 in human breast carcinoma tissue.
Flow Cytometry: A 1:30 dilution of a previous lot was used in flow cytometry.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human Her2.
Epitope
C-terminus
Clone
EP1045Y
Host
Rabbit
Specificity
This antibody detects Her2 phosphorylated at Tyr1248 as well as unphosphorlyated Her2.
FUNCTION:Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin. CATALYTIC ACTIVITY:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT STRUCTURE:Heterodimer with each of the other ERBB receptors Potential. Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248. Interacts with MUC1. Stimulation by heregulin (HRG) in breast cancer cell lines induces binding of MUC1 with gamma-catenin. SUBCELLULAR LOCATION:Membrane; Single-pass type I membrane protein.
PTM: Ligand-binding increases phosphorylation on tyrosine residues By similarity.
Polymorphism There are fours alleles due to the variations in positions 654 and 655. Allele B1 (Ile-654/Ile-655) has a frequency of 0.782; allele B2 (Ile-654/Val-655) has a frequency of 0.206; allele B3 (Val-654/Val-655) has a frequency of 0.012.
Involvement in disease Defects in ERBB2 are associated with gastric cancer [MIM:137215]; also known as hereditary familial diffuse gastric cancer (HDGC).
Defects in ERBB2 are associated with familial glioma of brain [MIM:137800]; also called glioblastoma multiforme. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas.
Defects in ERBB2 are associated with ovarian cancer [MIM:167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of visceral metastases. These typical features relate to the biology of the disease, which is a principal determinant of outcome.
Defects in ERBB2 are associated with lung cancer [MIM:211980]; also called adenocarcinoma of lung. SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on SKBR-3 cell lysates.
Western Blot Analysis: A 1:50,000-100,000 dilution of this antibody was used to detect ErbB2/Her2 in SKBR-3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
ErbB2/Her2 is a member of the ErbB receptor family of tyrosine kinases. ErbB2 can be activated without a ligand if it is overexpressed and can be detected in approximately 40% of human breast cancers. ErbB2 is a major target that is being explored In the treatment of breast cancer and other carcinomas.
Product Information
Format
Unpurified
Control
SKBR-3 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-ErbB2/Her2 Antibody, clone EP1045Y, Rabbit is validated for use in WB, FC, IH(P), IP for the detection of ErbB2/Her2.
Key Applications
Immunoprecipitation
Western Blotting
Flow Cytometry
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected ErbB2/Her2 in human breast carcinoma tissue.
Flow Cytometry: A 1:30 dilution of a previous lot was used in flow cytometry.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human Her2.
Epitope
C-terminus
Clone
EP1045Y
Host
Rabbit
Specificity
This antibody detects Her2 phosphorylated at Tyr1248 as well as unphosphorlyated Her2.
FUNCTION:Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin. CATALYTIC ACTIVITY:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT STRUCTURE:Heterodimer with each of the other ERBB receptors Potential. Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248. Interacts with MUC1. Stimulation by heregulin (HRG) in breast cancer cell lines induces binding of MUC1 with gamma-catenin. SUBCELLULAR LOCATION:Membrane; Single-pass type I membrane protein.
PTM: Ligand-binding increases phosphorylation on tyrosine residues By similarity.
Polymorphism There are fours alleles due to the variations in positions 654 and 655. Allele B1 (Ile-654/Ile-655) has a frequency of 0.782; allele B2 (Ile-654/Val-655) has a frequency of 0.206; allele B3 (Val-654/Val-655) has a frequency of 0.012.
Involvement in disease Defects in ERBB2 are associated with gastric cancer [MIM:137215]; also known as hereditary familial diffuse gastric cancer (HDGC).
Defects in ERBB2 are associated with familial glioma of brain [MIM:137800]; also called glioblastoma multiforme. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas.
Defects in ERBB2 are associated with ovarian cancer [MIM:167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of visceral metastases. These typical features relate to the biology of the disease, which is a principal determinant of outcome.
Defects in ERBB2 are associated with lung cancer [MIM:211980]; also called adenocarcinoma of lung. SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on SKBR-3 cell lysates.
Western Blot Analysis: A 1:50,000-100,000 dilution of this antibody was used to detect ErbB2/Her2 in SKBR-3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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