Nerve Growth Factor Receptor, also termed NGFR p75, p75NTR, or CD271 is the low affinity NGFR (LNGFR) which binds NGF and other neurotrophins including BDNF, NT3, NT4/5 with similar, low affinity. NGFR p75 is a 75 kDa transmembrane glycoprotein (399 a.a.) consisting of an extracellular domain (222 a.a.) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (22 a.a.), and a cytoplasmic domain (155 a.a.). NGFR p75 is mainly expressed in Schwann cells and neurons and in a variety of non neuronal cells. NGFR p75 is necessary for regulating neuronal growth, migration, differentiation and cell death during development of the central and peripheral nervous system. NGFR p75 plays a central role in the regulation of cell number by apoptosis in the developing CNS.
Product Information
Format
Unpurified
Control
PC12 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-NGF Receptor Antibody, clone EP1039Y, Rabbit detects level of NGF Receptor & has been published & validated for use in IH(P), WB, IC & IP.
Key Applications
Immunoprecipitation
Immunohistochemistry (Paraffin)
Western Blotting
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected NGF Receptor in human brain glioma tissue.
Immunocytochemistry Analysis: A 1:50 dilution from a previous lot was used in IC.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to residues of human NGF Receptor.
Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.
FUNCTION:Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT STRUCTURE:Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. Interacts with KIDINS220 and NTRK1. Can form a ternary complex with NTRK1 and KIDINS220 and this complex is affected by the expression levels of KIDINS220. An increase in KIDINS220 expression leads to a decreased association of NGFR and NTRK1(By similarity). Interacts with LINGO1 SUBCELLULAR LOCATION:Membrane; Single-pass type I membrane protein. DOMAIN:Death domain is responsible for interaction with RANBP9. The extracellular domain is responsible for interaction with NTRK1 By similarity. SEQUENCE SIMILARITIES:Contains 1 death domain. Contains 4 TNFR-Cys repeats.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on PC12 cell lysates.
Western Blot Analysis: 1:2,000-50,000 dilution of this antibody was used to detect NGF Receptor in PC12 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Nerve Growth Factor Receptor, also termed NGFR p75, p75NTR, or CD271 is the low affinity NGFR (LNGFR) which binds NGF and other neurotrophins including BDNF, NT3, NT4/5 with similar, low affinity. NGFR p75 is a 75 kDa transmembrane glycoprotein (399 a.a.) consisting of an extracellular domain (222 a.a.) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (22 a.a.), and a cytoplasmic domain (155 a.a.). NGFR p75 is mainly expressed in Schwann cells and neurons and in a variety of non neuronal cells. NGFR p75 is necessary for regulating neuronal growth, migration, differentiation and cell death during development of the central and peripheral nervous system. NGFR p75 plays a central role in the regulation of cell number by apoptosis in the developing CNS.
Product Information
Format
Unpurified
Control
PC12 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-NGF Receptor Antibody, clone EP1039Y, Rabbit detects level of NGF Receptor & has been published & validated for use in IH(P), WB, IC & IP.
Key Applications
Immunoprecipitation
Immunohistochemistry (Paraffin)
Western Blotting
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected NGF Receptor in human brain glioma tissue.
Immunocytochemistry Analysis: A 1:50 dilution from a previous lot was used in IC.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to residues of human NGF Receptor.
Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.
FUNCTION:Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT STRUCTURE:Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. Interacts with KIDINS220 and NTRK1. Can form a ternary complex with NTRK1 and KIDINS220 and this complex is affected by the expression levels of KIDINS220. An increase in KIDINS220 expression leads to a decreased association of NGFR and NTRK1(By similarity). Interacts with LINGO1 SUBCELLULAR LOCATION:Membrane; Single-pass type I membrane protein. DOMAIN:Death domain is responsible for interaction with RANBP9. The extracellular domain is responsible for interaction with NTRK1 By similarity. SEQUENCE SIMILARITIES:Contains 1 death domain. Contains 4 TNFR-Cys repeats.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on PC12 cell lysates.
Western Blot Analysis: 1:2,000-50,000 dilution of this antibody was used to detect NGF Receptor in PC12 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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