RIPAb+ Anti-SNRNP70

• 产品编号：merck-m-03-103      品牌：millipore       原厂货号：03-103
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Description:RIPAb+ SNRNP70 - RIP Validated Antibody and Primer Set.

Overview:RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully coprecipitating a specific RNA target (where such a specific target is known). The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. Each set also includes a negative control antibody to ensure specificity of the RIP reaction.
The RIPAb+ SNRNP70 set includes the SNRNP70 antibody, a negative control antibody (purified rabbit IgG), and positive qPCR primers which amplify a 100 bp fragment of the human cDNA of U1snRNA. The SNRNP70 and negative control antibodies are supplied in a scalable "per RIP" reaction size and can be used to functionally validate the precipitation of SNRNP70-associated RNA.

Presentation:Anti-SNRNP70 (Rabbit Polyclonal IgG)
One vial containing 50 μg of antibody lyophilized from PBS buffer with 2% sucrose. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL. For longer periods of storage store at -20°C. 

Normal Rabbit IgG
One vial containing 125 μg of purified rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.

RIP Primers, U1snRNA 
One vial containing 75 μL of 5 μM of each primer specific for the cDNA of U1 snRNP. Store at -20°C.
FOR: GGG AGA TAC CAT GAT CAC GAA GGT
REV: CCA CAA ATT ATG CAG TCG AGT TTC CC

Key Applications

• Western Blotting
• RNA Binding Protein Immunoprecipitation (RIP)
• Immunoprecipitation

原厂资料：

Description:RIPAb+ SNRNP70 - RIP Validated Antibody and Primer Set.

Overview:RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully coprecipitating a specific RNA target (where such a specific target is known). The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. Each set also includes a negative control antibody to ensure specificity of the RIP reaction.
The RIPAb+ SNRNP70 set includes the SNRNP70 antibody, a negative control antibody (purified rabbit IgG), and positive qPCR primers which amplify a 100 bp fragment of the human cDNA of U1snRNA. The SNRNP70 and negative control antibodies are supplied in a scalable "per RIP" reaction size and can be used to functionally validate the precipitation of SNRNP70-associated RNA.

Presentation:Anti-SNRNP70 (Rabbit Polyclonal IgG)
One vial containing 50 μg of antibody lyophilized from PBS buffer with 2% sucrose. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL. For longer periods of storage store at -20°C. 

Normal Rabbit IgG
One vial containing 125 μg of purified rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.

RIP Primers, U1snRNA 
One vial containing 75 μL of 5 μM of each primer specific for the cDNA of U1 snRNP. Store at -20°C.
FOR: GGG AGA TAC CAT GAT CAC GAA GGT
REV: CCA CAA ATT ATG CAG TCG AGT TTC CC

Key Applications

• Western Blotting
• RNA Binding Protein Immunoprecipitation (RIP)
• Immunoprecipitation

注意事项：

Application Notes

Immunoprecipitation from RIP lysate:
RIP lysate from HeLa cells (2 X 10⁶ cell equivalents per IP) was subjected to immunoprecipitation using 0.5 μg of either a normal rabbit IgG or Anti-SNRNP70 antibody. Precipitated proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SNRNP70 (1.0 μg/mL). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231) (Please see figures).