nHU VEGF(REC) 10UG

• 产品编号：merck-m-01-185      品牌：millipore       原厂货号：01-185
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > VEGF血管内皮生长因子
• 应用分类：

描述：

人重组血管内皮生长因子（VEGF），用于细胞培养。

原厂资料：

VEGF Protein, Human recombinant.

The human recombinant VEGF Protein is available in a 10 µg format.

注意事项：

VEGF Proliferation Assay

1. Prior to use in this assay, maintain HUVE cells in F-12K medium supplemented with 10% (v/v) FBS, 1% penicillin-streptomycin-glutamine, 0.1 mg/ml heparin and 0.05 mg/ml endothelial cell growth supplement (ECGS).

2. For assay of VEGF, subculture HUVE cells into a 96-well plate at a density of 4,000 cells per well in the growth medium over night.

3. Add VEGF to a final concentration range of 0.2, 0.4, 0.8, 1.6, 3.2, 6.2, 12.5, 25 and 50ng/ml. Add VEGF diluent to one set of wells as the negative control.
4. Four days later, add 20ul of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well.
5. Incubate at 37C for 2 hrs and read at OD 490nm.