HisLink? 96 Protein Purification System

产品编号：V3681 品牌：promega 原厂货号：V3681
产品分类：Discontinued已停产
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其它组分：

HisLink™ 96 Filtration/Collection Plates【子货号：V484A，包装：5 x 1 pack，，运保温度：室温】

Binding/Wash Buffer【子货号：V851D，包装：2 x 280ml，，运保温度：4°C】

Elution Buffer【子货号：V852D，包装：1 x 125ml，，运保温度：4°C】

FastBreak™ Cell Lysis Reagent, 10X【子货号：V857B，包装：1 x 60ml，，运保温度：室温】

HisLink™ Protein Purifiication Resin【子货号：V882A，包装：2 x 50ml，，运保温度：4°C】

DNase I (lyophilized)【子货号：Z358A，包装：1 x 1 vial，，运保温度：–20°C】

描述：

HisLink™ Protein Purification Systems are designed for purification of polyhistidine (His)-tagged or HQ-tagged proteins directly from culture medium containing bacterial cells expressing the tagged protein of interest. All product formats use the HisLink™ Protein Purification Resin (Cat.# V8821), a macroporous silica resin derivatized with a high level of tetradentate-chelated nickel (>20mmol Ni/ml settled resin). The resin performs well in either column, batch or vacuum-based methods with a binding capacity of >15mg/ml of resin. The HisLink™ Protein Purification Resin is useful in all general immobilized metal affinity chromatography (IMAC) applications matrix as well as in low- to medium-pressure liquid chromatography systems.

The HisLink™ 96 Protein Purification System (Cat.# V3680) provides a simple and quick vacuum-based method of simultaneously purifying multiple His-tagged proteins from E. coli cultured in deep-well, 96-well plates. The HisLink™ 96 System is amenable to manual or automated methods, such as the Beckman Coulter Biomek® 2000 or FX for high-throughput applications, yielding 1mg of purified polyhistidine-tagged protein per well.