The ELISA-Light™ Immunoassay System with CDP-Star® and Sapphire-II™ Substrate⁄Enhancer Solution is used for ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.
• Heterogeneous assay format increases sensitivity and dynamic range and reduces interference by test compounds in the detection portion of the assay (as compared with homogeneous assay formats) • Chemiluminescent immunoassay detection allows greater sensitivity and improved dynamic range as compared to radioimmunoassays and immunoassays that employ colorimetric or fluorescent detection • High sensitivity and wide dynamic range permit sensitive detection with fewer assays, because sample dilution to fit the dynamic range of the assay is unnecessary • A variety of ready-to-use substrate formulations lets the user precisely optimize signal intensity to the exact requirements of an assay • The kinetics of the glow-assay provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used • Chemiluminescent immunoassay detection provides versatile applications such as sandwich immunoassays, competitive immunoassays, and DNA probe capture assays • Non-radioactive immunoassays eliminate concerns over use of radioisotopes
Extend the Limits of Detection The ELISA-Light™ system offers an excellent combination of high sensitivity and throughput for assays measured in a luminometer. The ELISA-Light™ system significantly extends the limits of detection of most immunoassays previously performed with colorimetric and isotopic methods.
Multiple Substrate⁄Enhancer Formulations The kit contains reagents optimized for chemiluminescent ELISAs employing alkaline phosphatase labels and is available with a choice of ready-to-use chemiluminescent substrate formulations containing CSPD® or CDP-Star® substrate with Sapphire-II™ or Emerald-II™ luminescence enhancers. Multiple substrate⁄enhancer formulations provide options that let the user optimize specific assays or luminometers. The specific formulation that will provide the highest sensitivity for a specific assay can vary. Therefore, an ELISA sampler kit containing all four substrate⁄enhancer formulations is available to facilitate assay development.
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For Research Use Only. Not for use in diagnostic procedures.
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