SBFI- AM CELL PERMEANT

产品编号：S1263 品牌：invitrogen 原厂货号：S1263
产品分类：生化试剂 > 染料/染色剂/指示剂/底物 > 传感器和指示剂 > 离子指示剂&离子载体
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包装：

1 MG

运保温度:

Store at -20°C

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描述：

SBFI is a sodium-sensitive molecule used to estimate Na+ gradients in isolated mitochondria, to measure intracellular NA+ levels, to measure Na+ efflux in cells, and, in combination with other fluorescent indicators used to correlate changes in intracellular Na+ with Ca2+ and Mg2+concentrations, intracellular pH, and membrane potential. Although the selectivity of SBFI for Na+ is less than that of calcium indicators such as fura-2, it is sufficient for the detection of physiological concentrations of Na+ in the presence of other monovalent cations. The spectral response of SBFI upon ion binding permit excitation ratio measurements, and this indicator can be used with the same optical filters and equipment used for fura-2.

Fluorescent Ion Indicators Specifications:
• Label (Ex/Em): SBFI (~340,380/500 nm)
• Lyophilized product may be dissolved in DMSO for use
• Product is typically loaded into cells by adding the dissolved indicator to medium containing cells

Selectivity Considerations and Cell Loading Strategies
The dissociation constant (Kd) of SBFI for Na+ is 3.8 mM in the absence of K+, and 11.3 mM in solutions with a combined Na+ and K+ concentration of 135 mM (which approximates physiological ionic strength). SBFI is ~18-fold more selective for Na+ than for K+.

SBFI is available as both cell-impermeant acid salt (S1262) and as cell-permeant acetoxymethyl (AM) esters (S1263, S1264). The anionic acid forms can be loaded into cells using our Influx™ pinocytic cell-loading reagent (I14402, Chelators, Calibration Buffers, Ionophores and Cell-Loading Reagents—Section 19.8), or by microinjection, patch-pipette infusion or electroporation. For AM ester loading (Loading and Calibration of Intracellular Ion Indicators—Note 19.1), addition of the Pluronic® F-127 (P3000MP, P6866, P6867) or PowerLoad™ (P10020) dispersing agents as well as relatively long incubation times—up to four hours—are typically necessary.

注意事项：

For Research Use Only. Not for use in diagnostic procedures.