Background: ICAM-1/CD54
Intercellular adhesion molecule 1 (ICAM-1), also known as CD54, binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface.
原厂资料：
Product Summary:
The Quantikine Rat sICAM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat sICAM-1 levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant rat sICAM-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring rat sICAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural rat sICAM-1.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
47
39
47
Mean
87
271
617
80
307
560
Standard Deviation
4.3
13
29
7.6
19
35
CV%
4.9
4.8
4.7
9.5
6.2
6.2
Recovery:
The recovery of rat sICAM-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Serum (n=6)
103
83-119
Cell Culture Supernates (n=8)
99
88-104
EDTA Plasma (n=5)
103
91-117
Linearity:
To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of rat sICAM-1 in each matrix were diluted with Calibrator Diluent and then assayed.
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