Tumor Necrosis Factor Receptor-I (TNF RI, also known as p55/p60) is a high affinity receptor for TNF-alpha and -beta, potent mediators of multiple aspects of inflammatory immune responses. TNF RI, along with TNF RII, is a prototypic member of the TNF receptor superfamily and has been designated TNFRSF1A. All cell types are thought to express TNF RI, and most of the biological functions of TNF are mediated via TNF RI. Soluble forms of both TNF RI, generated as a result of proteolytic cleavage of the extracellular domain, has been detected in vivo in various biological fluids. The soluble TNF RI receptor can bind TNF with high affinity and functions as a TNF antagonist.
TNF RI is thought to form homotrimers or oligomers upon ligand binding. The TNF RI extracellular domain includes four cysteine-rich repeats characteristic of the TNF receptor superfamily. TNF RI is capable of interacting with adaptor proteins upstream of NF-kB activation and contains a cytoplasmic "death domain" upstream of caspase-dependent apoptosis cascades.
原厂资料：
Product Summary:
The Quantikine Mouse/Rat TNF RI immunoassay is a 4.5 hour solid-phase ELISA designed to measure TNF RI in mouse or rat cell culture supernates and serum. It contains E. coli-expressed recombinant mouse TNF RI and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse TNF RI. Results obtained using natural mouse or rat TNF RI showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural TNF RI.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
46
118
581
48.3
120
592
Standard Deviation
2.7
6.5
27.5
2.5
6
33.3
CV%
5.9
5.5
4.7
5.2
5
5.6
Recovery:
The recovery of TNF RI spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Mouse Cell Culture Supernates (n=5)
97
89-109
Rat Cell Culture Supernates (n=5)
98
92-116
Mouse Serum (n=5)
96
83-104
Rat Serum (n=5)
96
88-104
Linearity:
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TNF RI in each matrix were diluted with Calibrator Diluent and assayed.
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