Background:RGMs
The repulsive guidance molecule (RGM) family of molecules contains three uniquely structured GPI-linked glycoproteins that participate in a variety of activities. Termed RGM-A, -B and -C, they are 40-45 kDa in size, show generally nonoverlapping fetal expression patterns, and serve as BMP-4 receptors. RGM-A and -C are plasma membrane bound, while RGM-B is generally microsomal in location. RGM-A and -B are associated with neurons, while RGM-C regulates skeletal muscle and hepatocyte function.
原厂资料：
Product Summary:
The Quantikine Mouse/Rat RGM-A Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse and rat RGM-A in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse RGM-A and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural RGM-A showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural RGM-A.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of mouse/rat RGM-A spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Mouse Cell Culture Supernates (n=4)
101
91-112
Rat Cell Culture Supernates (n=4)
100
90-113
Mouse Tissue Homogenates (n=4)
100
93-106
Mouse Serum (n=4)
113
103-120
Rat Serum (n=4)
107
94-119
Mouse EDTA Plasma (n=4)
106
91-119
Rat EDTA Plasma (n=4)
107
103-115
Rat Heparin Plasma (n=4)
99
91-106
Mouse Heparin Plasma (n=4)
102
95-117
Linearity:
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of RGM-A were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
