描述：
Rat CCL2 is a member of the β (C-C) subfamily of chemokines that is a chemoattractant for
monocytes and basophils but not eosinophils or neutrophils. Rat CCL2 is secreted as a 14 kDa
glycoprotein monomer but noncovalent dimers probably occur. The first five amino acids of
the mature protein are essential for activity; deletion of the N-terminal glutamine, which is
pyrrolidone carboxylic acid-modified, dramatically decreases activity on basophils and,
surprisingly, stimulates eosinophil chemotaxis. The rat CCL2 propeptide shares 82% amino
acid (a.a.) identity with mouse CCL2 over the 148 a.a. sequence and 57%, 52%, 52%, 52% and
52% a.a. identity with equine, human, porcine, canine and guinea pig CCL2, respectively, over
the first 100 aa. Rat and mouse CCL2 have a 49 aa extension at the C-terminus as compared to
human CCL2. Fibroblasts, tumor cells, smooth muscle cells, endothelial cells, and mononuclear
phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation. CCL2 is best
known as a chemotactic agent for mononuclear cells. It also induces enzyme and cytokine
release by monocytes, NK cells and lymphocytes, and histamine release by basophils, primarily
due to interaction with CCR2 receptors on these cells. Additionally, it is believedto reduce IL12
production by dendritic cells and promote a Th2 phenotype in CD4+ T cells. The role of CCL2 in
recruiting monocytes to sites of inflammation is implicated in the pathology of multiple sclerosis,
atherosclerosis and allergic asthma.
原厂资料：
Rat CCL2 is a member of the β (C-C) subfamily of chemokines that is a chemoattractant for
monocytes and basophils but not eosinophils or neutrophils. Rat CCL2 is secreted as a 14 kDa
glycoprotein monomer but noncovalent dimers probably occur. The first five amino acids of
the mature protein are essential for activity; deletion of the N-terminal glutamine, which is
pyrrolidone carboxylic acid-modified, dramatically decreases activity on basophils and,
surprisingly, stimulates eosinophil chemotaxis. The rat CCL2 propeptide shares 82% amino
acid (a.a.) identity with mouse CCL2 over the 148 a.a. sequence and 57%, 52%, 52%, 52% and
52% a.a. identity with equine, human, porcine, canine and guinea pig CCL2, respectively, over
the first 100 aa. Rat and mouse CCL2 have a 49 aa extension at the C-terminus as compared to
human CCL2. Fibroblasts, tumor cells, smooth muscle cells, endothelial cells, and mononuclear
phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation. CCL2 is best
known as a chemotactic agent for mononuclear cells. It also induces enzyme and cytokine
release by monocytes, NK cells and lymphocytes, and histamine release by basophils, primarily
due to interaction with CCR2 receptors on these cells. Additionally, it is believedto reduce IL12
production by dendritic cells and promote a Th2 phenotype in CD4+ T cells. The role of CCL2 in
recruiting monocytes to sites of inflammation is implicated in the pathology of multiple sclerosis,
atherosclerosis and allergic asthma.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
