RXR ALPHA COACTIVATOR ASSAY

• 产品编号：PV4797      品牌：invitrogen       原厂货号：PV4797
• 产品分类：生化和细胞分析 > 生化分析试剂盒 > 核受体辅因子相互作用分析
• 应用分类：行业及应用 > 药物研发 > 靶标和先导药物鉴定及验证 > G 蛋白偶联受体生物学G-Protein Coupled Receptor Biology (GPCR) > Membrane Protein Expression System > 生物化学核受体分析 > 核受体分析 > LanthaScreen™ TR-FRET NR 辅调节因子相互作用分析

描述：

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15138:

The LanthaScreen® TR-FRET Retinoid X Receptor alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential RXR alpha ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti- GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant RXR alpha ligand-binding domain (RXR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format. 

To assay: 
When running the LanthaScreen® TR-FRET Retinoid X Receptor alpha Coactivator Assay in agonist mode (to identify agonist compounds), RXR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the TR-FRET ratio of 520/495 is calculated and can be used to determine the EC50 from a dose-response curve of the compound. Based on the biology of the RXR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (see Figure 1).

原厂资料：

注意事项：

For Research Use Only. Not for use in diagnostic procedures.