MCP-2 (Monocyte Chemoattractant Protein 2), otherwise known as CCL8, is a CC chemokine originally isolated from the human osteosarcoma cell line MG-63, which is expressed at highest levels in peripheral blood cells and the small intestine.
MCP-2 acts as a chemoattractant for monocytes, lymphocytes, eosinophils and basophils, and signals through binding to the common MCP receptor, chemokine (C-C motif) receptor 2 (CCR2), and also CCR1 and CCR3. Proteolytic cleavage of MCP-2 into the NH2-terminally truncated form, MCP-2 (6-76), results in the loss of chemotactic activity, forming a functional CC chemokine inhibitor, which may be important during inflammatory responses.
Target Species
Mouse
Product Form
Purified recombinant protein - lyophilised
Preparation
Purified recombinant MCP-2 expressed in E.coli
Preservative Stabilisers
None present
Molecular Weight
8.5kD (74 amino acid residues)
Reconstitution
Reconstitute with 200ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AbD Serotec recommend that the vial is gently mixed after reconstitution.
Purity
%98 by SDS PAGE and HPLC analysis
Activity
Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10.0-100.0 ng/ml.