Bone morphogenetic protein-2 (BMP-2), is a 26 kDa dimer consisting of 2 115 amino acid polypeptide chains and belongs to the TGF beta family of structurally related signalling proteins. It is primarily expressed in lung, spleen and colon but also at significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.
In association with osteoconductive carriers such as collagen and synthetic hydroxyapaptite, BMP-2 can induce bone and cartilage formation and is involved in cell proliferation and morphogenesis. The osteolytic activity of BMP-2 can be exploited by metastatic tumours and BMP-2 can also enhance angiogenesis. However BMP-2 can act as a tumour suppressor, promoting apoptosis in mature colonic epithelial cells and lowered BMP-2 expression is associated with prostate cancer progression.
Along with TGF-beta, BMP-2 activates both Ras/MAPK/AP-1 and Smad signalling in osteoblasts with Smads modulating AP-1 activity.
Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Preparation
Purified recombinant BMP-2, expressed in E. coli
Preservative Stabilisers
None present
Molecular Weight
26kD
Reconstitution
Reconstitute with 100ul distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Serotec recommend that the vial is gently mixed after reconstitution.
Purity
>98% by SDS PAGE and HPLC analysis.
Activity
The ED50 as determined by its ability to induce alkaline phosphatase production by ATDC-5 cells is < 1µg/ml.