MCA1053G recognises to the 45kD leucocyte surface glycoprotein specific for the CD48 antigen which is closely related to the activation antigen, Blast-1.
It is useful in the detection of CD48 positive cells and may be used in studies to differentiate haemapoietic tumours, sarcomas and melanomas.
Species Cross-Reactivity
Target Species
Cross Reactivity
Rhesus Monkey
Yes
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
1/50 - 1/100
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting(1)
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
(1)
This product recognizes CD48 under non-reducing conditions.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
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