RIBOMINUS(BACTERIA)

产品编号：K155004 品牌：invitrogen 原厂货号：K155004
产品分类：DNA和RNA纯化 > RNA纯化 > RNA纯化
应用分类：核酸分离纯化与分析 > RNA纯化 > 按RNA类型 > 转录组RNA

包装：

12 REACTIONS

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其它组分：

RiboMinus™ Magnetic Beads (12 mg/ml) in Phosphate Buffered Saline (PBS), pH 7.4 containing 0.01% Tween 20 and 0.09% sodium azide【包装：3 ml】

RiboMinus™ Bacteria Probes in ultrapure water (100 pmol/μl)【包装：50 μl】

Hybridization Buffer (B10)【包装：6 ml】

RNase-Free Water【包装：6 ml】

描述：

RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5µ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods. The RiboMinus™ Transcriptome Isolation Kits enable:

• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods

• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)

查看更多Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.

注意事项：

The RiboMinus™ Isolation Kits include RiboMinus™ Magnetic Beads, RiboMinus™ human/mouse, bacteria, or yeast probe, and Hybridization Buffer. The optional RiboMinus™ Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water. Store the isolation modules at +4°C and the concentration module at room temperature. All reagents are guaranteed stable for 6 months when properly stored.