MITOCHONDRIAL PERMEABILITY TRANSITION KIT

The MitoPT^TM JC-1 kit uses a quick and easy staining method to clearly differentiate between non-apoptotic and apoptotic cells through mitochondrial functionality.

MitoPT^TM JC-1 Reagent - lyophilized
10X Assay Buffer, 30mL
125uL vial 50mM Carbonylcyanide m - chlorophenylhydrazone (CCCP) concentrate in DMSO.

An early indication of apoptosis involves a collapse in the electrochemical gradient across the mitochondrial membrane. Loss of mitochondrial membrane potential can be detected by a unique fluorescent cationic dye known as JC-1 (5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzamidazolocarbocyanin iodide) that has been incorporated into the MitoPT^TM JC-1 kit.

The MitoPT^TM JC-1 reagent easily penetrates cells and enters the mitochondria. It aggregates in the mitochondria of non-apoptotic cells and fluoresces red, whilst in apoptotic cells it diffuses throughout the cell. Once dispersed, the reagent assumes a monomeric form and fluoresces green. This allows an easy distinction between non-apoptotic red fluorescent cells and apoptotic green fluorescent cells which can be read with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.

Store the unopened kit (and each unopened component) at +4oC until the expiration date. Protect the MitoPT^TMJC-1 reagent from light at all times.

Once reconstituted, the 100X MitoPT^TMJC-1 stock should be stored at -20oC protected from light.

The 100X MitoPT^TM JC-1 reconstituted stock is stable for up to 6 months and may be thawed twice during that time.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions for use can be found at www.abdserotec.com/uploads/IFU/ICT943.pdf

Material Safety Datasheet documentation #20291; #10474; #10479 available at
http://www.abdserotec.com/uploads/MSDS/20291.pdf
http://www.abdserotec.com/uploads/MSDS/10474.pdf
http://www.abdserotec.com/uploads/MSDS/10479.pdf

MitoPT^TM is a trademark of Immunochemistry Technologies, LLC.

For research purposes only