The Magic Red™Cathepsin K kit uses a quick and easy method to analyze intracellular Cathepsin K protease activity in whole living cells.
Reagents In The Kit
Magic Red™MR-(LR)2Reagent - lyophilized
Hoechst 33342 Stain, 1 mL
Acridine orange (AO), 0.5 mL
Test Principle
Cathepsin Magic Red™kits measure cathepsin K protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product.
The Magic Red™reagent contains a cathepsin K target sequence peptide (LR)2linked to a red (Cresyl Violet) fluorescent probe.
The Cathepsin Magic Red™kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.
This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
Storage
Store the unopened kit (and each unopened component) at +4oC until the expiration date.
Protect the MR-(LR)2reagent from light at all times.
Once reconstituted, the 260X MR-(LR)2stock should be stored at -20°C protected from light.
Shelf Life
The 260X MR-(LR)2reconstituted stock is stable for up to 6 months and may be thawed twice during that time.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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