HCA150A recognises Human PTEN-induced putative Kinase protein 1 (PINK1), a 581 aa single pass membrane protein belonging the Serine/Threonine protein kinase.
PINK1 protects against mitochondrial dysfunction during cellular stress, possibly by phosphorylating mitochondrial proteins. PINK1 is highly expressed in tissues such as the heart, skeletal muscle and testis. Lower expression levels are detected in the brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine.
Defects in PINK1 are the cause of autosomal recessive early-onset Parkinson disease 6 (PARK6).
Removal of sodium azide is recommended prior to use in functional assays – AbD Serotec recommend the use of EQU003 for this purpose.
Product Form
A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed inE. coli.. The antibody is tagged with a V5 tag and a Strep tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
1:50 -
Immunohistology - Paraffin(1)
1:50 -
Immunoprecipitation
Western Blotting
2ug/ml -
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
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