HCA149A recognizes human DJ-1, also known widely as PARK7, a nuclear and cytoplasmic protein of approximately 21kDa belonging to the C56 peptidase family.
DJ-1 plays roles in transcriptional regulation and reaction to anti-oxidative stress. Research indicates that DJ-1 is responsible for protecting neurones against oxidative stress and cell death. De-regulation of DJ-1 oxidation appears to be connected to the onset of conditions such as Parkinson's Disease.
Research also suggests that the oxidation status of DJ-1, particularly at C106, modulates the function of DJ-1, AbD Serotec have also introduced an antibody that specifically recognises DJ-1 when oxidized at C106 (product code HCA124).
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed inE. coli.This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with V5 and a Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
1:1000 - 1:2000
Immunoprecipitation
Western Blotting
2ug/ml -
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
This product does not require protein digestion pre-treatment of paraffin sections. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose.
Histology Positive Control Tissue
Human kidney
Western Blotting
HCA149A detects a band of approximately 21kDa in HeLa cell lysates.
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