Clone 7627 recognizes bovine FoxP3, a 48kD forkhead transcription factor family member, which was originally identified as the novel protein scurfin. FoxP3 functions as a DNA binding protein that represses transcription, and is reported to be involved in the regulation of T cell activation, differentiation and homeostasis.
FoxP3 is predominantly expressed within the nuclei of CD25 +CD4 + regulatory T cells (Tr). This population of FoxP3+CD25+CD4+ Tr cells plays an essential role in controlling autoimmunity.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit
www.abdserotec.com/protocols.
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Application Name |
Yes |
No |
Not Determined |
Suggested Dilution |
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ELISA |
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Flow Cytometry(1) |
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Functional Assays |
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Immunohistology - Frozen |
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Immunohistology - Paraffin |
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Immunoprecipitation |
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Western Blotting |
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Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
(1)
Membrane permeabilization is required for this application. AbD Serotec recommends the use of 0.1% Triton X-100 for this purpose.
Technical Advice
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
ELISA
This product is suitable for use in direct ELISA applications. Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA,
please enquire.
UniProt
Health And Safety Information
Acknowledgements
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by AbD Serotec.
Regulatory
For research purposes only
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