HCA094 recognises myelin proteolipid protein (PLP), which is a major constituent protein of central nervous system myelin. The Plp gene is highly conserved across species and encodes two major membrane-bound proteins of the myelin sheath, the large isoform PLP and a smaller isoform DM20. The exact functions of PLP are still unclear but reports suggest that PLP and DM20 may play a role in the formation of the intraperiod line of myelin and in maintaining axonal integrity.
In man, mutations of the PLP and DM20 proteins have been reported which are associated with X-linked dysmyelinating disorders including the heterogeneous syndromes of Pelizaeus-Merzbacher disease (PMD). The reported mutations vary considerably and range from deletions, missense mutations to additional copies of the gene.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Dog
Expected from Sequence
Mouse
Expected from Sequence
Pig
Expected from Sequence
Rabbit
Expected from Sequence
Rat
Expected from Sequence
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed inE. coli.This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by AbD Serotec.
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