HCA063 recognises human cyclin-dependent kinase 2 (Cdk2), a member of the serine/threonine kinase family of cyclin-dependent kinases. Cyclin-dependant kinases (CDKs) are a class of enzymes that control cell cycle progression in a variety of organisms. Cdk2 forms active heterodimeric complexes with regulatory subunits, including cyclin A or cyclin E, resulting in the phosphorylation of the retinoblastoma tumour suppressor protein. Its activity is regulated by phosphorylation and CDK inhibitors p21/WAF1 (CDKN1A) and p27/kip1 (CDKN1B).
Cdk2 is involved in the regulation of G1/S transition and S phase progression of the cell cycle but may also play a role in apoptosis and DNA double strand break repair following ionising radiation.
Cdk2 can be up-regulated in tumours, including adenomas and breast cancers, particularly in conjunction with cyclin over-expression.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed inE. coli.This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/100 - 1/500
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
HCA063 detects a band of approximately 35kDa in HCT-116 cell lysates.
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by AbD Serotec.
Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA,please enquire.
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