HCA053 recognises the Ki67 cell-cycle assocaited protein. Ki-67 is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki-67 gene contains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis reveals multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quiescent cells. Immunoblot analysis shows expression of 320- and 359-kD proteins. Antisense oligonucleotides inhibit cellular proliferation in a dose-dependent manner, suggesting that Ki-67 protein expression may be an absolute requirement for cell proliferation.
Within cells Ki67 is predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Chimpanzee
Expected from Sequence
Dog
Expected from Sequence
Macaque
Expected from Sequence
Rhesus Monkey
Expected from Sequence
Product Form
Chimeric human-mouse IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. The antibody has variable regions of human origin and an Fc portion (including CH1 and CL domains) from mouse IgG1. It can be detected by anti mouse Fc specific secondary antibodies. This antibody is supplied lyophilised.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
2ug/ml
Immunohistology - Frozen
Immunohistology - Paraffin(1)
1/100 - 1/500
Western Blotting
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA,please enquire.
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