HCA004 recognises human CD292, also known as Bone Morphogenetic Protein Receptor type 1A (BMPR-1A) and does not cross-react with human BMPR-1B.
The Bone Morphogenetic Protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR-1A and BMPR-1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. BMPR-1A is the receptor for BMP-2 and BMP-4. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kDa and type II receptors of about 70-80 kDa. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signalling, whereas type I receptors require their respective type II receptors for ligand binding.
In humans, mutations in the BMPR-1A gene are responsible for juvenile polyposis syndrome, juvenile intestinal polyposis, and Cowden disease.
HCA004 has been shown to inhibit BMP-2 stimulation of C2C12 cells.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Dog
Expected from Sequence
Mouse
Yes
Product Form
Monovalent human recombinant Fab selected from the HuCAL®GOLD phage display library. Expressed inE. coliand purified using Streptactin affinity chromatography. The antibody is tagged with a strepII-tag (NWSHPQFEK) at the C-terminus of the antibody heavy chain-liquid.
Activity
Activity was tested by indirect ELISA: recombinant purified BMPR-1A (5 µg/ml) plus unrelated control proteins were immobilized on a microtiter plate. Specific binding was monitored by first adding HCA004 (2 µg/ml), then adding a secondary antibody (goat anti-human F(ab')2 fragment specific, AP conjugate, 1:5000 diluted). A fluorescent signal was created by adding the AP substrate Attophos. The signal on the antigen is at least 5-fold above background, whereas the signal on the control antigens is less than 1.5-fold above back-ground.
Affinity
kD = 2.2 nM
The monovalent intrinsic affinity of HCA004 was measured by BIAcore on the immobilized BMPR-1A extracellular domain.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
2ug/ml
Functional Assays
Western Blotting
2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by AbD Serotec.
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents.
This antibody was developed byAbD Serotec, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
Blot data kindly provided by Professor Sebald, University of Wuerzburg, Germany.
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