The FluoroSpot assay for Interferon-gamma (IFN-γ) and Interleukin-5 (IL-5) enables simultaneous detection of distinct cell populations secreting either of these two cytokines, as well as the prevalence of possible co-secreting cells.
FluoroSpot kit for the simultaneous detection of mouse IFN-γ and IL-5. The complete kit contains low fluorescent PVDF-based membrane plates, capture mAbs, detection mAbs, fluorophore-conjugated secondary detection reagents, anti-CD28 mAb and Fluorescence enhancer-II.