F10-89-4 recognises the human leucocyte common antigen (CD45), which is expressed by all haematopoietic cells. In peripheral blood the antigen is expressed more strongly by lymphocytes than by granulocytes. Erythrocytes do not express CD45.
UCHM1 recognises a 55kDa glycoprotein expressed on the surface of monocytes and macrophages, and belongs to the CD14 cluster. Weak expression of CD14 may be detected on peripheral blood granulocytes.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry
Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
