NCBP1/CBP80 Antibody

• 产品编号：CST-9983S      品牌：Cell Signaling Technology       原厂货号：9983S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NCBP1/CBP80 Antibody能够检测内源性的 NCBP1/CBP80 总蛋白。该多克隆抗体是利用合成的对应于人NCBP1/CBP80蛋白Val452位点周围序列的肽段免疫动物而生产的。经过Protein A和亲和层析纯化。Nuclear cap-binding protein subunit 1 (NCBP1), 也称之为cap-binding protein 80 (CBP80)在pre-mRNA剪接中发挥作用（1,2）。研究显示它还在mRNA的无义降解 (NMD)中执行功能，在这里翻译被过早的终止了（3）。NCBP1/CBP80能够促进NMD两个活性组分Upf1和Upf2的相互作用从而提高NMD的效率（4）。

1. Izaurralde, E. et al. (1994) Cell 78, 657-68.
2. Izaurralde, E. et al. (1995) Nature 376, 709-12.
3. Ishigaki, Y. et al. (2001) Cell 106, 607-17.
4. Hosoda, N. et al. (2005) Nat Struct Mol Biol 12, 893-901.

原厂资料：

Specificity / Sensitivity

NCBP1/CBP80 recognizes endogenous levels of total NCBP1/CBP80 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val452 of human NCBP1/CBP80 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Nuclear cap-binding protein subunit 1 (NCBP1), also known as cap-binding protein 80 (CBP80), plays a role in nuclear pre-mRNA splicing (1,2). It has also been shown to function in the nonsense-mediated decay (NMD) of mRNAs where translation is prematurely terminated (3). NCBP1/CBP80 increases the efficiency of NMD by promoting the interaction of two active NMD components Upf1 and Upf2 (4).

1. Izaurralde, E. et al. (1994) Cell 78, 657-68.
2. Izaurralde, E. et al. (1995) Nature 376, 709-12.
3. Ishigaki, Y. et al. (2001) Cell 106, 607-17.
4. Hosoda, N. et al. (2005) Nat Struct Mol Biol 12, 893-901.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.