IKK Isoform Antibody Sampler Kit

• 产品编号：CST-9966S      品牌：Cell Signaling Technology       原厂货号：9966S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

多克隆抗体是通过合成从人的IKKβ, IKKε and IKKγ C端到IKKα N端20个氨基酸处的肽段来免疫动物而获得的。抗体通过protein A和亲和层析法纯化。IKK Isoform Antibody Sampler Kit 为我们研究胞内NFkappaB信号通路提供了经济快速的方法。本试剂盒内含四个Western blots 所需的一抗和二抗。NF-κB/Rel转录调控因子在细胞质中与IκB抑制蛋白结合以非活性形式存在(1-3)。大部分激活因子采用通常信号通路——即磷酸化诱导、蛋白水解酶体介导降解IκB的方法激活NF-κB (3-7)。此通路中最重要的步骤是激活高分子量的IκB激酶（IKK）复合体，此复合体催化的反应中有三个紧密相关的IKK亚基参与完成，IKKα and IKKβ 作为激酶的催化单元，IKKγ作为调控单元(8,9)。IKK的激活依赖于特定位点的磷酸化：IKKβ活性环中的 Ser177和Ser181位点以及IKKα中的serine 176 和 180 位点，这些位点的磷酸化引起蛋白构象的变化从而激活激酶（10-13）。

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Zandi, E. et al. (1997) Cell 91, 243-52.
9. Karin, M. (1999) Oncogene 18, 6867-74.
10. DiDonato, J.A. et al. (1997) Nature 388, 548-54.
11. Mercurio, F. et al. (1997) Science 278, 860-6.
12. Johnson, L.N. et al. (1996) Cell 85, 149-58.
13. Delhase, M. et al. (1999) Science 284, 309-13.

原厂资料：

Specificity / Sensitivity

IKKα, IKKβ, IKKγ, and IKKε antibodies detect endogenous levels of total IKKα, IKKβ, IKKγ and IKKε proteins, respectively. These antibodies do not cross-react with IKK subunits other than their specified target.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxyl terminus of human IKKβ, IKKε and IKKγ, to the 20 amino-terminal residues of human IKKα. Antibodies are purified by protein A and peptide affinity chromatography.

Description

The IKK Isoform Antibody Sampler Kit provides an economical means to investigate NFkappaB signaling within the cell. The kit contains primary and secondary antibodies to perform four Western blots with each antibody.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation; Ser177 and Ser181 in the activation loop of IKKβ (serine 176 and 180 in IKKα) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13).

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Zandi, E. et al. (1997) Cell 91, 243-52.
9. Karin, M. (1999) Oncogene 18, 6867-74.
10. DiDonato, J.A. et al. (1997) Nature 388, 548-54.
11. Mercurio, F. et al. (1997) Science 278, 860-6.
12. Johnson, L.N. et al. (1996) Cell 85, 149-58.
13. Delhase, M. et al. (1999) Science 284, 309-13.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.