Cadherin-Catenin Antibody Sampler Kit

产品编号：CST-9961S 品牌：Cell Signaling Technology 原厂货号：9961S
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

1 Kit

运保温度:

–20°C

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其它组分：

N-Cadherin (D4R1H) XP® Rabbit mAb 【子货号：#13116，包装：40 µl，，运保温度：–20°C】

P-Cadherin (C13F9) Rabbit mAb 【子货号：#2189，包装：40 µl，，运保温度：–20°C】

E-Cadherin (24E10) Rabbit mAb 【子货号：#3195，包装：40 µl，，运保温度：–20°C】

α-E-Catenin (23B2) Rabbit mAb【子货号： #3240，包装：40 µl，，运保温度：–20°C】

Pan-Cadherin (28E12) Rabbit mAb【子货号： #4073，包装：40 µl，，运保温度：–20°C】

Anti-rabbit IgG, HRP-linked Antibody 【子货号：#7074，包装：100 µl，，运保温度：–20°C】

β-Catenin (D10A8) XP® Rabbit mAb 【子货号：#8480，包装：40 µl，，运保温度：–20°C】

描述：

Cadherin-Catenin抗体样品试剂盒中的每个抗体识别它的特异靶标，不与其他家族成员交叉反应，Pan-Cadherin (28E12)兔源单抗#4073识别内源总的cadherin蛋白。多克隆抗体通过合成肽段免疫动物产生，肽段与人β-catenin 的C末端和人N-cadherin里的残基相对应，由蛋白A和肽段亲和层析技术纯化。单克隆抗体由合成肽段免疫动物产生，肽段分别对应人α-E-catenin的N末端序列，人P-cadherin的C末端附近残基，人E-cadherin的780附近残基，并且人N-, R-, E-和P-Cadherin保守区域与合成肽段对应。Cadherin-Catenin抗体样品试剂盒含试剂检测细胞粘着连接中发现的关键蛋白的总蛋白水平，试剂盒含有足够的一抗和二抗，可做四次Western blot实验。粘着连接是一种形成细胞与细胞接触的动态结构，在发育、分化、组织完整性、形态学和细胞极性。它们由钙粘着糖蛋白这类跨膜蛋白组成，这些蛋白以钙依赖的方式在粘附细胞上结合钙粘着糖蛋白。粘着连接的细胞质那一边，钙粘着糖蛋白与β-catenin, γ-catenin 和 p120 catenin (δ)相关联，β-catenin 和 γ-catenin与α-catenin相关，α-catenin连接cadherin-catenin复合物到actin细胞骨架（1,2）。近来的研究显示，癌症表现增加的N-cadherin和减少的E-cadherin表达，E-cadherin被认为是一种侵袭癌症细胞生长的抑制因子，这种在cadherin表达上的变化与癌症发展相关，被命名为“cadherin开关”，β-catenin是Wnt信号通路下游关键效应因子之一，并牵涉早期胚胎发育和肿瘤形成（3-5）。

原厂资料：

Specificity / Sensitivity

Each antibody in the Cadherin-Catenin Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Pan-Cadherin (28E12) Rabbit mAb #4073 detects endogenous levels of total cadherin proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human β-catenin and residues within human N-cadherin and are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human α-E-catenin, residues near the carboxy terminus of human P-cadherin, residues surrounding 780 of human E-cadherin, and a synthetic peptide corresponding to a conserved region of human N-, R-, E- and P-Cadherin.

Description

This Cadherin-Catenin Antibody Sampler kit contains reagents to examine the total protein levels of key proteins found in cell-cell adherens junctions. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.

Background

Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of cadherins that are transmembrane proteins that bind cadherins on adjacent cells in a calcium dependent manner. On the cytoplasmic side of adherens junctions, the cadherins associate with β-catenin, γ-catenin and p120 catenin (δ). β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Recent studies indicate that cancer cells exhibit increased N-cadherin and diminished E-cadherin expression. E-cadherin is considered a suppressor of invasive cancer cell growth and this change in cadherin expression associated with cancer progression is termed the “cadherin switch”. β-catenin is one of the key downstream effectors in the Wnt signaling pathway and has been implicated in early embryonic development and tumorigenesis (3-5).

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5),
150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at
–20°C. Do not aliquot the antibodies