Jak Isoform Sampler Kit

• 产品编号：CST-9945S      品牌：Cell Signaling Technology       原厂货号：9945S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

每一个Jak蛋白异形体抗体只能识别内源性特异的Jak蛋白异形体且不依赖于磷酸化状态。每一个抗体都不与其余两个异形体抗体交叉反应。每一个亚型特异性抗体都是以人工合成特异的Jak不同亚型的多肽免疫动物而产生。Jak1和Jak3抗体通过protein A和多肽亲和层析法纯化。试剂盒中每一个Jak抗体都是特异性的均不与其余异形体抗体交叉反应。Jak Isoform Antibody Sampler Kit 为几个Jak亚型的研究提供了经济的方法。试剂盒中包含了足够做四次Western免疫印迹的一抗和二抗。Janus家族中的酪氨酸激酶成员(Jak1, Jak2, Jak3和Tyk2)通过配体结合到一些相关的细胞因子受体上而被激活（1）。除了细胞因子受体的激活方式，Jak蛋白能自身磷酸化和磷酸化与其相关的受体从而为信号蛋白提供多个结合位点。这些信号蛋白如Stats (2), Shc (3), 胰岛素受体底物(4) and 成簇黏附激酶 (FAK) (5)都是典型的含有SH2 或者其它的磷-酪氨酸结合区域。

1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
2. Darnell, J.E. (1997) Science 277, 1630-5.
3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.

原厂资料：

Specificity / Sensitivity

Each Jak isoform antibody recognizes endogenous levels of the specified Jak isoform independent of its phosphorylation state. Neither antibody cross-reacts with the two other isoforms.

Source / Purification

Each isoform-specific antibody is produced by immunizing animals with a synthetic peptide that is specific for the Jak isoform. Jak1 and Jak3 antibodies are purified by protein A and peptide affinity chromatography. Each Jak antibody included in this kit is specific and does not cross-react with the other isoforms.

Description

The Jak Isoform Antibody Sampler Kit provides an economical means to examine several Jak isoforms. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
2. Darnell, J.E. (1997) Science 277, 1630-5.
3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibodies.