Apoptosis Antibody Sampler Kit

• 产品编号：CST-9915S      品牌：Cell Signaling Technology       原厂货号：9915S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Apoptosis Antibody Sampler Kit中的每一种抗体检测各自对应内源性蛋白。Cleaved Caspase-3 (Asp175)、Cleaved Caspase-7 (Asp198) 和Cleaved PARP (Asp214) 抗体只能够检测它们各自蛋白的裂解大片段。Cleaved Caspase-9 (Asp330)抗体能够检测相应蛋白的37 kDa(with prodomain)或17 kDa的裂解大片段。 Caspase-3、Caspase-7、Caspase-9 和PARP 抗体都能够检测它们各自蛋白的全长和裂解大片段。这些单克隆抗体和多克隆抗体分别是用合成的人源caspase-3、-7和-9，及人PARP蛋白裂解位点周围氨基序列对应的肽段免疫动物而制备。多克隆抗体是通过蛋白A和肽段亲和层析来纯化。Apoptosis Antibody Sampler Kit 提供了较为经济的方法检测活化和未活化caspases。该试剂盒包含了需要一抗和二抗的量，每种抗体足够四次Western blot实验。细胞凋亡是一个被调控的生理过程，导致细胞死亡。Caspases，是一个半胱氨酸蛋白酶家族，是细胞凋亡的中心调控因子。引发剂 caspases（包括8、9、10和12）被紧密耦合到促凋亡信号。一旦被激活，这些 caspases 切割并激活下游的效应 caspases（包括3、6和7），它们反过来切割细胞骨架和核蛋白质，如PARP、α-胞衬蛋白、DFF和 lamin A，并诱导细胞凋亡。从线粒体释放的细胞色素c耦合到活化的caspase-9，这是一种胱天蛋白酶引发剂（1）。促凋亡刺激物包括FasL、TNF - α、DNA损伤和内质网应激。Fas和TNFR激活 caspases 8和10（2），DNA损伤导致caspase - 9的活化，同时ER应激导致钙离子介导的caspase-12活化（3）。凋亡蛋白抑制子（IAP）家族包括XIAP和Survivin，通过结合及抑制 caspases 发挥功能（4,5）。线粒体应激时，线粒体蛋白Smac/Diablo被释放到细胞质，与半胱天冬酶竞争结合IAPs。Smac/Diablo和IAPs的相互作用解除了IAPs对半胱天冬酶的抑制效应（6）。

1. Baker, S.J. and Reddy, E.P. (1998) Oncogene 17, 3261-3270.
2. Budihardjo, I. et al. (1999) Annu. Rev. Cell Dev. Biol. 15, 269-290.
3. Nakagawa, T. et al. (2000) Nature 403, 98-103.
4. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
5. Li, F. et al. (1998) Nature 396, 580-584.
6. Du, C. et al. (2000) Cell 102, 33-42.

原厂资料：

Specificity / Sensitivity

Each antibody in the Apoptosis Antibody Sampler Kit detects endogenous levels of its respective protein. Cleaved Caspase-3 (Asp175), Cleaved Caspase-7 (Asp198) and Cleaved PARP (Asp214) Antibodies recognize only the large fragments of their respective cleaved proteins. Cleaved Caspase-9 (Asp330) Antibody recognizes either the 37 kDa (with prodomain) or 17 kDa large fragment of the cleaved protein. Caspase-3, Caspase-7, Caspase-9 and PARP Antibodies recognize both the full length and large cleaved fragments of their respective proteins.

Source / Purification

Monoclonal and polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding the proteolytic cleavage sites of human caspase-3, -7 and -9 and human PARP. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Apoptosis Antibody Sampler Kit provides an economical means to evaluate the levels of inactive and active caspases. The kit contains enough primary and secondary antibodies to perform four Western blot experiments with each antibody.

Background

Apoptosis is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Initiator caspases (including 8, 9, 10 and 12) are closely coupled to proapoptotic signals. Once activated, these caspases cleave and activate downstream effector caspases (including 3, 6 and 7), which in turn cleave cytoskeletal and nuclear proteins like PARP, α-fodrin, DFF and lamin A, and induce apoptosis. Cytochrome c released from mitochondria is coupled to the activation of caspase-9, a key initiator caspase (1). Proapoptotic stimuli include the FasL, TNF-α, DNA damage and ER stress. Fas and TNFR activate caspases 8 and 10 (2), DNA damage leads to the activation of caspase-9 and ER stress leads to the calcium-mediated activation of caspase-12 (3). The inhibitor of apoptosis protein (IAP) family includes XIAP and survivin and functions by binding and inhibiting several caspases (4,5). Smac/Diablo, a mitochondrial protein, is released into the cytosol upon mitochondrial stress and competes with caspases for binding of IAPs. The interaction of Smac/Diablo with IAPs relieves the inhibitory effects of the IAPs on caspases (6).

1. Baker, S.J. and Reddy, E.P. (1998) Oncogene 17, 3261-3270.
2. Budihardjo, I. et al. (1999) Annu. Rev. Cell Dev. Biol. 15, 269-290.
3. Nakagawa, T. et al. (2000) Nature 403, 98-103.
4. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
5. Li, F. et al. (1998) Nature 396, 580-584.
6. Du, C. et al. (2000) Cell 102, 33-42.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5),
150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at
–20°C. Do not aliquot the antibody.