β-Amyloid (D12B2) Rabbit mAb

• 产品编号：CST-9888S      品牌：CST       原厂货号：9888S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

β-Amyloid (D12B2) Rabbit mAb兔单抗能够检测内源性的β-淀粉样肽(Aβ)。该抗体检测Aβ多种亚型，如Aβ-37，Aβ-38，Aβ-39，Aβ-40和Aβ-42。该抗体的抗原与大鼠并非100%保守。然而，该抗体能够在检测小鼠Aβ的同时，小鼠和大鼠抗原周围侧翼序列也100%会被检测到。该单克隆抗体是由合成的人源β-淀粉样肽(Aβ)N末端肽段免疫动物而制备的。β淀粉样蛋白（Amyloid β, Aβ）前体蛋白（Amyloid β precursor protein, APP）是分子量为100-140 kDa的跨膜糖蛋白，它拥有几个亚型（1）。APP的氨基酸序列中所包含的淀粉样蛋白域（A-β），能够通过两步蛋白裂解反应被释放出来（1）。释出的A-β片段在细胞外沉积积累，形成了阿尔茨海默病(Alzheimer's disease，AD)中淀粉样斑块的主要组成部分（1）。APP存在多个能够被磷酸化的位点，这些位点影响着蛋白本身的裂解和分泌过程（2-5）。细胞周期蛋白依赖性激酶介导的Thr688（对应于APP695亚型）磷酸化是细胞周期依赖的，在G2/M期达到峰值（4）。APP的Thr668磷酸化形式存在于成年大鼠大脑和相应的培养神经细胞及其分化细胞中（5,6）。

1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
2. Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.
3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.
4. Suzuki, T. et al. (1994) EMBO J 13, 1114-22.
5. Ando, K. et al. (1999) J Neurosci 19, 4421-7.
6. Iijima, K. et al. (2000) J Neurochem 75, 1085-91.

原厂资料：

Specificity / Sensitivity

β-Amyloid (D12B2) Rabbit mAb recognizes endogenous levels of total β-amyloid (Aβ) peptide. The antibody detects several isoforms of Aβ, such as Aβ-37, Aβ-38, Aβ-39, Aβ-40, and Aβ-42. The antigen for this antibody is not 100% conserved in rat. However, the antibody detects mouse Aβ and the mouse and rat sequences surrounding the antigen are 100% identical.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Aβ peptide.

Background

Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (AD) (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).

1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
2. Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.
3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.
4. Suzuki, T. et al. (1994) EMBO J 13, 1114-22.
5. Ando, K. et al. (1999) J Neurosci 19, 4421-7.
6. Iijima, K. et al. (2000) J Neurochem 75, 1085-91.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody