Phospho-Numb (Ser276) (D5C2) Rabbit mAb

• 产品编号：CST-9878S      品牌：CST       原厂货号：9878S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Numb (Ser276) (D5C2) Rabbit mAb兔单抗只识别内源性的Numb蛋白。单克隆抗体是通过一种合成的磷酸化肽段去免疫动物产生。这种合成的肽段与邻近人源Numb蛋白的Ser276氨基酸残基序列一致。Numb包含一个氨基末端磷酸化酪氨酸结合（PTB）结构域和C末端对α-衔接蛋白和EH（Eps15 同源物）结构域包含蛋白的内吞结合基序，显示在细胞吞噬中的作用（1,2）有四个哺乳动物Numb剪接异构体，差异表达，并有各自独特功能（3-5）。Numb通过促进Notch泛素降解对Notch信号通路起负向调控作用（6），蛋白在细胞分裂时不对称的入子细胞，产生的两个子细胞对Notch信号和细胞命运都有不同反应（7,8）。Numb可能被G蛋白偶联受体和PKC信号调控（9）。Numb的不同位点可以被磷酸化，包括Ser7, Ser276和Ser295。在这些位点的磷酸化可调控Numb的不对称膜定位和整合内吞作用。

1. Berdnik, D. et al. (2002) Dev. Cell 3, 221-231.
2. Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352.
3. Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104.
4. Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476.
5. Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155.
6. McGill, M.A. and McGlade, C.J. (2003) J. Biol. Chem. 278, 23196-23203.
7. Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145.
8. Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948.
9. Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.
10. Nishimura, T. and Kaibuchi, K. (2007) Dev Cell 13, 15-28.
11. Smith, C.A. et al. (2007) EMBO J 26, 468-80.
12. Wirtz-Peitz, F. et al. (2008) Cell 135, 161-73.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Chicken, Xenopus, Zebrafish, Bovine, Horse

Specificity / Sensitivity

Phospho-Numb (Ser276) (D5C2) Rabbit mAb recognizes endogenous levels of numb protein only when phosphorylated at Ser276.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser276 of human numb protein.

Background

Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling (9).Numb can be phosphorylated at several sites including Ser7, Ser276, and Ser295. Phosphorylation at these sites regulates asymmetric membrane localization of Numb and integrin endocytosis (10-12).

1. Berdnik, D. et al. (2002) Dev. Cell 3, 221-231.
2. Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352.
3. Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104.
4. Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476.
5. Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155.
6. McGill, M.A. and McGlade, C.J. (2003) J. Biol. Chem. 278, 23196-23203.
7. Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145.
8. Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948.
9. Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.
10. Nishimura, T. and Kaibuchi, K. (2007) Dev Cell 13, 15-28.
11. Smith, C.A. et al. (2007) EMBO J 26, 468-80.
12. Wirtz-Peitz, F. et al. (2008) Cell 135, 161-73.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody