Cyclin Antibody Sampler Kit

• 产品编号：CST-9869S      品牌：Cell Signaling Technology       原厂货号：9869S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

描述：

Cyclin Antibody Sampler Kit 试剂盒中的每个抗体都能够检测其内源性靶蛋白，且不与其他周期蛋白发生交叉反应。单克隆抗体是由合成的针对cyclin D1蛋白羧基末端的肽段、cyclin D2蛋白氨基末端肽段、重组的人cyclin E和cyclin A蛋白免疫动物生产的。多克隆抗体是由合成的针对cyclin H, cyclin E2蛋白羧基末端肽段, cyclin B蛋白氨基末端肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。.Cyclin Antibody Sampler Kit能够经济地评价细胞中细胞周期蛋白的存在。该试剂盒内含足够4次western blot实验的一抗和二抗。细胞周期调节受众多的细胞事件和进程控制。细胞周期蛋白依赖性激酶(CDK)调节这些途径，并结合其细胞周期蛋白伙伴，构成一个活化的复合体。该活性主要通过磷酸化来控制，从而决定了CDK/细胞周期蛋白复合物的亚细胞定位(1,2)。有些磷酸化事件控制细胞质滞留序顺序的功能，而其它事件调节核定位和输出顺序功能(3,4)。细胞周期蛋白和细胞周期蛋白依赖性激酶抑制剂(CKI)水平受泛素化和泛素蛋白酶体降解途径的调节(5)。各种CKI蛋白质联合这些复合物并调节-细胞周期蛋白调节结构域的进入(6)。控制随细胞周期阶段振的细胞周期蛋白水平将产生额外的复杂性。细胞周期蛋白D1表达的增加与某些类型的癌症相关(7,8)，且可能联合TSC2（薯球蛋白）而不依赖其Cdk伴侣(9)。

原厂资料：

Specificity / Sensitivity

Each antibody in the Cyclin Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other cyclin proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues within the carboxy terminus of cyclin D1, the amino terminus of cyclin D2, recombinant human cyclin E, or cyclin A. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues within the carboxy terminus of cyclin H, cyclin E2, or the amino terminus of cyclin B. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Cyclin Antibody Sampler Kit provides an economical means of evaluating the presence of cyclin proteins in cells. The kit contains enough primary and secondary antibodies to perform four western blot experiments with each antibody.

Background

Control of the cell cycle is regulated by a multitude of cellular events and processes. The cyclin dependent kinases (CDK) regulate many of these pathways and constitute an active complex when associated with their cyclin partners. This activity is controlled primarily by phosphorylation, which determines subcellular localization of the CDK/cyclin complex (1,2). Some phosphorylation events control the function of cytoplasmic retention sequences while other events regulate nuclear localization and export sequence function (3,4). Cyclin and cyclin-dependent kinase inhibitor (CKI) levels are regulated by ubiquitination and degradation via the ubiquitin proteasome pathway (5). A variety of CKI proteins associate with these complexes and modulate access to regulatory domains on cyclins (6). Additional complexity is generated as the controlled protein levels of each cyclin oscillate with the stages of cell cycle. Increased expression of Cyclin D1 is associated with certain types of cancer (7,8) and may associate with TSC2 (tuberin) independent of its Cdk partner (9). 

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.