HtrA2/Omi (D20A5) Rabbit mAb

• 产品编号：CST-9745S      品牌：CST       原厂货号：9745S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HtrA2/Omi (D20A5) Rabbit mAb兔单抗能够检测内源性的HtrA2/Omi 总蛋白。该抗体不会与HtrA1产生交叉反应。 该单克隆抗体是采用合成的人源HtrA2/Omi蛋白Phe341周围残基相对应的肽段免疫动物而制备的。蛋白A2（HtrA2）/Omi是一种丝氨酸蛋白酶，与大肠杆菌的HtrA蛋白(DegP) 同源，研究表明其参与细胞凋亡及蛋白错误折叠引起的降解等过程（1）。最初的研究表明HtrA2存在于细胞核（1）或粗面内质网（2）中，后来的研究证明HtrA2存在于线粒体上，在细胞凋亡过程中被释放出来（3-8）。HtrA2产生时是50 kDa的酶原，之后被裂解成36kDa的成熟蛋白，暴露出一个氨基末端基序（AVPS），AVPS类似于IAP抑制子Smac/Diablo（3-8）。与SMAC一样，HtrA2和IAP家族成员之间的相互作用，如XIAP，拮抗caspase活性的抑制，保护细胞免于凋亡（3-8）。有趣的是，在HtrA2基因敲除小鼠模型中，并没有显示出细胞凋亡的减少，而是出现了纹状体中的神经元缺失和帕金森样表型，这表明HtrA2可能具有神经保护功能（9-11）。该活性与HtrA2的蛋白酶活性有关（9）。此外，研究已经在帕金森病中发现了HtrA2基因功能缺失的突变体（12）。

1. Gray, C.W. et al. (2000) Eur. J. Biochem. 267, 5699-5710.
2. Faccio, L. et al. (2000) J. Biol. Chem. 275, 2581-2588.
3. Suzuki, Y. et al. (2001) Mol. Cell 8, 613-621.
4. Hegde, R. et al. (2002) J. Biol. Chem. 277, 432-438.
5. Martins, L.M. et al. (2002) J. Biol. Chem. 277, 439-444.
6. van Loo, G. et al. (2002) Cell Death Differ. 9, 20-26.
7. Verhagen, A.M. et al. (2002) J. Biol. Chem. 277, 445-454.
8. Jones, J.M. et al. (2003) Nature 425, 721-727.
9. Vaux, D.L. and Silke, J. (2003) Cell 115, 251-253.
10. Martins, L.M. et al. (2004) Mol. Cell Biol. 24, 9848-9862.
11. Strauss, K.M. et al. (2005) Hum. Mol. Genet. 14, 2099-2111.

原厂资料：

Specificity / Sensitivity

HtrA2/Omi (D20A5) Rabbit mAb recognizes endogenous levels of total HtrA2/Omi protein. This antibody does not cross-react with HtrA1.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe341 of human HtrA2/Omi protein.

Background

High temperature requirement protein A2 (HtrA2)/Omi is a serine protease with homology to the E. coli HtrA protein (DegP) and is thought to be involved in apoptosis and stress-induced degradation of misfolded proteins (1). While HtrA2 was orignally identified to be present in either the nucleus (1) or endoplasmic reticulum (2), subsequent studies have shown that it localizes in mitochondria and is released during apoptosis (3-8). HtrA2 is produced as a 50 kDa zymogen that is cleaved to generate a 36 kDa mature protein that exposes an amino terminal motif (AVPS) resembling that of the IAP inhibitor Smac/Diablo (3-8). Like Smac, interaction between HtrA2 and IAP family members, such as XIAP, antagonizes their inhibition of caspase activity and protection from apoptosis (3-8). Interestingly, HtrA2 knock-out mice did not show signs of reduced apoptosis, but rather had a loss of neurons in the striatum and a Parkinson's-like phenotype, suggesting that HtrA2 might have a neuroprotective function (9-11). This activity is associated with the protease activity of HtrA2 (9). Furthermore, research studies have shown that loss of function mutations in the HtrA2 gene are associated with Parkinson's disease (12).

1. Gray, C.W. et al. (2000) Eur. J. Biochem. 267, 5699-5710.
2. Faccio, L. et al. (2000) J. Biol. Chem. 275, 2581-2588.
3. Suzuki, Y. et al. (2001) Mol. Cell 8, 613-621.
4. Hegde, R. et al. (2002) J. Biol. Chem. 277, 432-438.
5. Martins, L.M. et al. (2002) J. Biol. Chem. 277, 439-444.
6. van Loo, G. et al. (2002) Cell Death Differ. 9, 20-26.
7. Verhagen, A.M. et al. (2002) J. Biol. Chem. 277, 445-454.
8. Jones, J.M. et al. (2003) Nature 425, 721-727.
9. Vaux, D.L. and Silke, J. (2003) Cell 115, 251-253.
10. Martins, L.M. et al. (2004) Mol. Cell Biol. 24, 9848-9862.
11. Strauss, K.M. et al. (2005) Hum. Mol. Genet. 14, 2099-2111.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.