Cleaved DFF45 (Asp224) Antibody

• 产品编号：CST-9731S      品牌：CST       原厂货号：9731S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

该抗体能够检测天冬氨酸224处裂解的DFF45羧基末端片段, 不能识别全长DFF45及其他DFF45裂解产物。该多克隆抗体是用合成的人DFF45 224位天冬氨酸C-末端序列相对应的肽段免疫的动物而制备获得。抗体经过了蛋白A和肽亲和层析技术进行纯化。在正常细胞中，在 caspase 激活的脱氧核苷酸酶（ (DFF40 or CAD) 合成过程中，人源DFF45和小鼠同源的ICAD作为分子伴侣共同发挥作用（1）。DFF45 (或同源的 DFF35)与DFF40联合抑制DFF40的活性（1-4)。体外试验中，研究证明 DFF45 包括caspase-3、-6、-7和-8等caspas家族和端粒酶B的靶点（3）。体内试验研究认为，caspas-3是主要的DFF45处理酶并释放其羧基末端片段(3,5)。DFF45的裂解阻止了其对DFF40的抑制作用，造成核DNA被DFF40裂解，最终导致细胞死亡(6,7).

1. Enari, M. et al. (1998) Nature 391, 43-50.
2. Sakahira, H. et al. (1998) Nature 391, 96-99.
3. Wolf, B. B. et al. (1999) J. Biol. Chem. 274, 30651-30656.
4. Gu, J. et al. (1999) J. Biol. Chem. 274, 20759-20762.
5. Tang, D. and Kidd, V.J. (1998) J. Biol. Chem. 273, 28549-28552.
6. Liu, X. et al. (1999) J. Biol. Chem. 274, 13836-13840.
7. Zhang, J. et al. (1999) J. Biol. Chem. 274, 37450-37454.

原厂资料：

Specificity / Sensitivity

Cleaved DFF45 (Asp224) Antibody detects the carboxy-terminal fragment of DFF45 resulting from cleavage at aspartic acid 224. The antibody does not recognize full length DFF45 or other cleavage products of DFF45.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp224 of human DFF45. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Human DFF45 and its mouse homologue ICAD function in normal cells as chaperones for caspase-activated deoxyribonuclease (DFF40 or CAD) during its synthesis (1). The association of DFF45 (or its isoform DFF35) with DFF40 inhibits the DNAse activity of the latter (1-4). In vitro, DFF45 has been shown to be the target of several caspases, including caspase-3, -6, -7, -8 and granzyme B (3). In vivo, caspase-3 is believed to be the primary enzyme responsible for processing DFF45 and release of its carboxy-terminal fragment (3,5). The cleavage of DFF45 inactivates its inhibitory function on DFF40 and causes nuclear DNA degradation by DFF40, leading to cell death (6,7).

1. Enari, M. et al. (1998) Nature 391, 43-50.
2. Sakahira, H. et al. (1998) Nature 391, 96-99.
3. Wolf, B. B. et al. (1999) J. Biol. Chem. 274, 30651-30656.
4. Gu, J. et al. (1999) J. Biol. Chem. 274, 20759-20762.
5. Tang, D. and Kidd, V.J. (1998) J. Biol. Chem. 273, 28549-28552.
6. Liu, X. et al. (1999) J. Biol. Chem. 274, 13836-13840.
7. Zhang, J. et al. (1999) J. Biol. Chem. 274, 37450-37454.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.