Integrin β1 (D2E5) Rabbit mAb

• 产品编号：CST-9699S      品牌：CST       原厂货号：9699S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Integrin β1 (D2E5) Rabbit mAb用于检测内源性的整合素β1总蛋白。该单克隆抗体由β1整合素Pro680周围残基合成的多肽免疫动物产生。整合素是细胞表面的α/β异二聚体受体，在细胞粘附、迁移、生长和存活中均起关键作用(1,2)。至少18种α亚基和8种β亚基，形成24种已知的整合素分布在不同的组织并具有交叉的配体特异性(3)。整合素不仅对细胞外环境信号应答并给细胞传输信号（从外向内传导），而且感知细胞内环境从而改变与细胞外环境的相互作用（由内向外传导）(1,2)。β1亚家族包括与不同的细胞外基质分子结合的12种不同的整合素蛋白(4)。控制细胞外整合素的结合可以影响细胞粘附和迁移，然而β1胞质尾区通过细胞外信号传递，从而有助于调节细胞增殖、细胞骨架的重组以及基因表达细胞外信号传递(4)。研究表明β1整合素在胚胎发育，血管、皮肤、骨骼及肌肉形成，以及肿瘤转移和血管形成中起作用(4,5)。

1. Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571.
2. Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100.
3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298.
4. Brakebusch, C. and Fässler, R. (2005) Cancer Metastasis Rev. 24, 403-411.
5. Mettouchi, A. and Meneguzzi, G. (2006) Eur. J. Cell Biol. 85, 243-247.

原厂资料：

Specificity / Sensitivity

Integrin β1 (D2E5) Rabbit mAb detects endogenous levels of total integrin β1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro680 of human integrin β1 protein.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). 
 The β1 subfamily includes 12 distinct integrin proteins that bind to different extracellular matrix molecules (4). Control of extracellular integrin binding influences cell adhesion and migration, while intracellular signaling messages relayed by the β1 cytoplasmic tail help to regulate cell proliferation, cytoskeletal reorganization, and gene expression (4). Research studies have implicated β1 integrin in various activities including embryonic development, blood vessel, skin, bone, and muscle formation, as well as tumor metastasis and angiogenesis (4,5).

1. Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571.
2. Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100.
3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298.
4. Brakebusch, C. and Fässler, R. (2005) Cancer Metastasis Rev. 24, 403-411.
5. Mettouchi, A. and Meneguzzi, G. (2006) Eur. J. Cell Biol. 85, 243-247.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody