PIP4K2B Antibody

• 产品编号：CST-9694S      品牌：CST       原厂货号：9694S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PIP4K2B抗体识别内源性的全部PIP4K2B蛋白。该多克隆抗体用与人类PIP4K2B中氨基末端区域附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。磷脂酰肌醇--磷酸 4-激酶（PIP4K）合成磷脂酰肌醇-4,5-二磷酸（PtdIns(4,5)P2），一种磷酸肌醇信号的关键前体，也直接调节某些蛋白质和细胞过程。PIP激酶有两个亚族，I和II，可以从不同的底物池中产生PtdIns(4,5)P2。PIP4KI型使用PtdIns5P作为底物，PIP5KII型激酶使用PtdIns4P作为底物（1,2）。在哺乳动物细胞中，每个PIP4K和PIP5K亚族还有三种亚型，分别由不同的基因编码（3-7）。所有PIPK激酶均被磷脂酸激活，被ARF和Rho GTP酶广泛调节，并被蛋白激酶A和PI激活的自身磷酸化所抑制（8）。

1. Rameh, L.E. et al. (1997) Nature 390, 192-6.
2. Zhang, X. et al. (1997) J Biol Chem 272, 17756-61.
3. Ishihara, H. et al. (1996) J Biol Chem 271, 23611-4.
4. Loijens, J.C. and Anderson, R.A. (1996) J Biol Chem 271, 32937-43.
5. Ishihara, H. et al. (1998) J Biol Chem 273, 8741-8.
6. Itoh, T. et al (1998) J. Biol. Chem. 273, 20292-20299
7. Boronenkov, I.V. et al. (1998) Mol Biol Cell 9, 3547-60.
8. Oude Weernink, P.A. et al. (2004) Eur J Pharmacol 500, 87-99.

原厂资料：

Specificity / Sensitivity

PIP4K2B Antibody recognizes endogenous levels of total PIP4K2B protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the residues of human PIP4K2B. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Phosphatidylinositol-5-phosphate 4-kinases (PIP4K) synthesize phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2), a key precursor in phosphoinositide signaling that directly modulates the activity of signaling proteins and cellular processes. There are two subfamilies of PIP kinases, type I and II, that generate PtdIns(4,5)P2 from distinct substrate pools. PIP4 type I kinases use PtdIns5P as a substrate, whereas PIP5 type II kinases use PtdIns4P (1,2). In mammalian cells, three isoforms of each PIP4K and PIP5K subfamily, encoded by distinct genes, have been characterized (3-7). All PIP kinases are stimulated by phosphatidic acid, extensively regulated by ARF and Rho GTPases, and inhibited by protein kinase A and PI-stimulated autophosphorylation (8).

1. Rameh, L.E. et al. (1997) Nature 390, 192-6.
2. Zhang, X. et al. (1997) J Biol Chem 272, 17756-61.
3. Ishihara, H. et al. (1996) J Biol Chem 271, 23611-4.
4. Loijens, J.C. and Anderson, R.A. (1996) J Biol Chem 271, 32937-43.
5. Ishihara, H. et al. (1998) J Biol Chem 273, 8741-8.
6. Itoh, T. et al (1998) J. Biol. Chem. 273, 20292-20299
7. Boronenkov, I.V. et al. (1998) Mol Biol Cell 9, 3547-60.
8. Oude Weernink, P.A. et al. (2004) Eur J Pharmacol 500, 87-99.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.