Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-9669S      品牌：CST       原厂货号：9669S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor^® 488 Conjugate)能够检测活化的邻近Asp175位点剪切后产生的 caspase-3 大片段 (17/19 kDa)产物。这种抗体不能识别全长的caspase-3 或其他的caspase裂解产物。偶联的Alexa Fluor^® 488荧光素激发光谱为 494 nm，发射光谱为 520 nm.该多克隆抗体是采用人源caspase-3 蛋白Asp175剪切位点周围残基相对应的肽段免疫动物后制备获得。抗体通过蛋白A和肽段亲和色谱纯化。在最佳条件下，抗体与Alexa Fluor^® 488耦合，F/P 比为2-6。该CST抗体与Alexa Fluor^® 488荧光染料耦合，可以用于人体细胞直接流式和免疫荧光分析的内部测试。该抗体与未结合染料的Cleaved Caspase-3 (Asp175) Antibody #9661 存在同源种属交叉反应。Caspase-3 (CPP-32, Apoptain, Yama, SCA-1)是一个凋亡的关键执行者，因为它对许多关键的蛋白起着部分或全部的裂解作用，比如核酶多聚(ADP-ribose)聚合酶(PARP)（1）。caspase-3的活化需要经过其未激活的的酶原转变成活化的 p17 和 p12 片段的蛋白水解过程。caspase-3的剪切需要天门冬氨酸位于p1位点（2）。

1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig

Specificity / Sensitivity

Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor^® 488 Conjugate) detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to aspartic acid 175. The antibody does not recognize full length caspase-3 or other cleaved caspases. Alexa Fluor^® 488 conjugates are excited at 494 nm and emit at 520 nm.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3. Antibodies are purified by protein A and peptide affinity chromatography. The antibody was conjugated to Alexa Fluor^® 488 under optimal conditions with an F/P ratio of 2-6.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-3 (Asp175) Antibody #9661.

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).

1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

注意事项：

Storage: Supplied in PBS (pH 7.2), less then 0.1% Sodium
azide, 2 mg/ml BSA. Store at 4°C. Protect from light.
Do not freeze.