14-3-3 ε Antibody

• 产品编号：CST-9635S      品牌：CST       原厂货号：9635S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

14-3-3 ε Antibody检测内源性14-3-3 ε总蛋白。通过人工合成人源14-3-3 ε蛋白相应的多肽片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。14-3-3蛋白家族在信号转导、关卡调控 （ checkpoint control）、凋亡和养分敏感通路中起着关键性调节作用(1,2)。14-3-3蛋白是高度保守且广泛表达的蛋白。至少七种亚型包括β、γ、ε、σ、ζ、τ和η ，它们都在哺乳动物体内被鉴定。最初描述的α和δ 亚型是分别被确认为 β和ζ亚型的磷酸化形式(3)。通过它们的氨基末端α 螺旋区域，14-3-3蛋白形成同源或异源二聚体，这些二聚体与很多的蛋白质包括转录因子、新陈代谢酶、细胞支架 蛋白、激酶、磷酸化酶和其它信号分子相互作用(3,4)。主要通过phospho-Ser/Thr motif促使14-3-3蛋白与它们的靶蛋白相互作用。然而，结合不同的phospho-Ser/Thr motifs和非磷酸化依赖的相互作用一样都已经被观察(4)。14-3-3蛋白的结合掩饰了其靶蛋白特异性序列，因此，该蛋白调节靶蛋白的定位、磷酸化水平、稳定性和分子相互作用(1-4)。14-3-3蛋白也可能诱导靶蛋白构象的改变，从而可调节靶蛋白功能(4,5)。明显的14-3-3蛋白亚型的时空表达模式在对细胞外信号和药物的发展和急性期已经被观察，这就认为尽管14-3-3蛋白亚型的序列相似，但是可能具有不同的功能(4)。数个研究认为14-3-3 蛋白亚型在癌症和神经综合征中不同的被调节(2,3)。

1. Muslin, A.J. and Xing, H. (2000) Cell Signal 12, 703-9.
2. Mackintosh, C. (2004) Biochem J 381, 329-42.
3. Dougherty, M.K. and Morrison, D.K. (2004) J Cell Sci 117, 1875-84.
4. Yaffe, M.B. (2002) FEBS Lett 513, 53-7.
5. Bridges, D. and Moorhead, G.B. (2004) Sci STKE 2004, re10.

原厂资料：

Specificity / Sensitivity

14-3-3 ε Antibody detects endogenous levels of total 14-3-3 ε protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human 14-3-3 ε. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed (4). 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes which modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).

1. Muslin, A.J. and Xing, H. (2000) Cell Signal 12, 703-9.
2. Mackintosh, C. (2004) Biochem J 381, 329-42.
3. Dougherty, M.K. and Morrison, D.K. (2004) J Cell Sci 117, 1875-84.
4. Yaffe, M.B. (2002) FEBS Lett 513, 53-7.
5. Bridges, D. and Moorhead, G.B. (2004) Sci STKE 2004, re10.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody