Slug (C19G7) Rabbit mAb

• 产品编号：CST-9585S      品牌：CST       原厂货号：9585S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Slug (L40C6)Rabbit mAb兔单抗识别内源性的总Slug蛋白。此单抗由重组的人源Slug蛋白免疫动物产生。Slug (SNAI2)是一类广泛表达的转录抑制因子，也是Snail家族锌指转录因子成员[1]。.与Snail蛋白相似，Slug蛋白在发育过程中结合E-钙黏着蛋白启动子区域抑制其转录[2]。Slug结合整合素蛋白启动子片段并抑制其表达，导致了细胞粘附减弱[3]。胚胎发育过程中发生EMT时E-钙黏着蛋白下调表达[4,5]。肿瘤抑制因子p53在γ射线照射过的细胞诱导Slug表达；Slug通过抑制p53诱导的Bcl-2促凋亡家族蛋白Puma的转录保护损伤的细胞免于凋亡[6]。缺失突变的相应Slug基因与色素沉淀异常综合症以及花斑有关。但是基因复制导致的Slug过表达却和先天性心脏缺损即法洛四联症有关[7]。

1. Inukai, T. et al. (1999) Mol Cell 4, 343-52.
2. Bolós, V. et al. (2003) J Cell Sci 116, 499-511.
3. Turner, F.E. et al. (2006) J Biol Chem 281, 21321-31.
4. Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-61.
5. Castro Alves, C. et al. (2007) J Pathol 211, 507-15.
6. Wu, W.S. et al. (2005) Cell 123, 641-53.
7. Pérez-Mancera, P.A. et al. (2006) Cytogenet Genome Res 114, 24-9.

原厂资料：

Specificity / Sensitivity

Slug (C19G7) Rabbit mAb detects endogenous levels of total Slug protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human Slug protein.

Background

Slug (SNAI2) is a widely expressed transcriptional repressor and member of the Snail family of zinc finger transcription factors (1). Similar to the related Snail protein, Slug binds to the E-cadherin promoter region to repress transcription during development (2). The binding of Slug to integrin promoter sequences represses integrin expression and results in reduced cell adhesion (3). Down regulation of E-cadherin expression occurs during the epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (4,5). The tumor suppressor protein p53 induces Slug expression in γ-irradiated cells; Slug protects damaged cells from apoptosis by repressing p53-induced transcription of the proapoptotic Bcl-2 family protein Puma (6). Deletion mutations in the corresponding Slug gene are associated with the pigmentation disorders Waardenburg Syndrome and Piebaldism, while a genetic duplication resulting in Slug overexpression is associated with a collection of congenital heart defects termed tetralogy of Fallot (7).

1. Inukai, T. et al. (1999) Mol Cell 4, 343-52.
2. Bolós, V. et al. (2003) J Cell Sci 116, 499-511.
3. Turner, F.E. et al. (2006) J Biol Chem 281, 21321-31.
4. Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-61.
5. Castro Alves, C. et al. (2007) J Pathol 211, 507-15.
6. Wu, W.S. et al. (2005) Cell 123, 641-53.
7. Pérez-Mancera, P.A. et al. (2006) Cytogenet Genome Res 114, 24-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.