PTEN (138G6) Rabbit mAb (Biotinylated)

• 产品编号：CST-9583S      品牌：CST       原厂货号：9583S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

PTEN (138G6) Rabbit mAb (Biotinylated)兔单抗可以检测内源性的总PTEN蛋白。单克隆抗体是采用人类PTEN蛋白羧基末端序列相对应残基的合成肽段免疫动物生产的。CST公司的本抗体在最佳条件下结合了生物素。非偶联的PTEN (26H9) Mouse mAb #9556可以与人类，小鼠、大鼠、猴子和仓鼠的PTEN发生反应。CST公司推测PTEN (138G6) Rabbit mAb (Biotinylated)兔单抗也识别以上物种。PTEN（phosphatase and tensin homologue deleted on chromosome ten），也称为MMAC（ (mutated in multiple advanced cancers)磷酸酶，是一种存在于多种人类癌症中的肿瘤抑制因子（1）。PTEN编码一个403个氨基酸组成的多肽，最初被认为是一个双特异性蛋白磷酸酶（2）。PTEN的主要底物是3磷酸肌醇激酶（PI3K）激活转化生成的肌醇磷脂（3）。PTEN是PI3K/Akt信号通路的一个主要的负调因子（1,4,5）。PTEN有一个羧基端的非催化调控的结构域，其中含有3个可以调节PTEN的稳定性，也可能影响其生物活性的磷酸化位点（Ser380，Thr382，Thr383）（6,7）。PTEN调节p53蛋白水平和活性（8），并在趋化过程中参与G蛋白偶联信号转导（9,10）。

1. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
2. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
3. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
4. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
5. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
6. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
7. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
9. Funamoto, S. et al. (2002) Cell 109, 611-23.
10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

原厂资料：

Specificity / Sensitivity

PTEN (138G6) Rabbit mAb (Biotinylated) detects endogenous levels of total PTEN protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human PTEN.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The unconjugated PTEN (26H9) Mouse mAb #9556 reacts with human, mouse, rat, monkey and hamster PTEN. CST expects that PTEN (138G6) Rabbit mAb (Biotinylated) will also recognize PTEN in these species.

Background

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).

1. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
2. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
3. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
4. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
5. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
6. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
7. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
9. Funamoto, S. et al. (2002) Cell 109, 611-23.
10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 2 mg/ml BSA, 50% glycerol. Store at -20ºC.
Do not aliquot antibody.