Cleaved PARP (Asp214) Antibody (Mouse Specific)

• 产品编号：CST-9544S      品牌：CST       原厂货号：9544S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cleaved PARP (Asp214) 抗体能够检测内源的caspase裂解的小鼠 PARP1(89 kDa)的大片段。该抗体与PARP1全长蛋白及其它PARP亚型不发生交叉反应。该多克隆抗体是采用合成的小鼠 Asp214 C-末端残基序列相对应的肽段免疫动物后制备获得。抗体通过蛋白A和肽段亲和色谱纯化。PARP，是核多聚(ADP-核糖) 聚合酶，分子量116kDa，参与环境胁迫应答中的DNA修复(1)。在体外该蛋白可被许多ICE样 caspases裂解(2,3)，在体内是caspase-3裂解的主要靶蛋白之一(4,5)。在人体中PARP在位点Asp214 和 Gly215被裂解，裂解后PARR的N末端DNA 结合结构域(24 kDa)与C端催化结构域(89 kDa) 分离(2,4)。PARP协助细胞维持生存能力；PARP的裂解促进细胞崩解并可以作为细胞凋亡的标记物(6）。

1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
2. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
3. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
4. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
5. Tewari, M. et al. (1995) Cell 81, 801-809.
6. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

原厂资料：

Specificity / Sensitivity

Cleaved PARP (Asp214) Antibody (Mouse Specific) detects endogenous levels of the large fragment (89 kDa) of mouse PARP1 resulting from caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp214 in mouse PARP. Antibodies are purified by protein A and peptide affinity chromatography.

Background

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
2. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
3. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
4. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
5. Tewari, M. et al. (1995) Cell 81, 801-809.
6. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody